Mining exosomal MicroRNAs from human-induced pluripotent stem cells-derived cardiomyocytes for cardiac regeneration

Sang Ging Ong, Won Hee Lee, Yang Zhou, Joseph C. Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Myocardial infarction is the leading cause of morbidity and mortality worldwide. Recent advances in cardiac regenerative therapy have allowed for novel modalities in replenishing the damaged myocardium. However, poor long-term engraftment and survival of transplanted cells have largely precluded effective cell replacement. As an alternative to direct cell replacement, the release of paracrine protective factors may be a more plausible effector for cardioprotection which may partially be mediated through secretion of microvesicles, or exosomes, that contribute to cell-cell communication. In this chapter, we describe the isolation of exosomes from induced pluripotent stem cells-derived cardiomyocytes for subsequent microRNA profiling for a better understanding of the biological cargo contained within exosomes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages127-136
Number of pages10
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1733
ISSN (Print)1064-3745

Keywords

  • Exosomes
  • Heart
  • iPSC-CMs
  • microRNA
  • Stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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