Mining the plant-herbivore interface with a leafmining Drosophila of Arabidopsis

Noah K Whiteman, Simon C. Groen, Daniela Chevasco, Ashley Bear, Noor Beckwith, T. Ryan Gregory, Carine Denoux, Nicole Mammarella, Frederick M. Ausubel, Naomi E. Pierce

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Experimental infections of Arabidopsis thaliana (Arabidopsis) with genomically characterized plant pathogens such as Pseudomonas syringae have facilitated the dissection of canonical eukaryotic defence pathways and parasite virulence factors. Plants are also attacked by herbivorous insects, and the development of an ecologically relevant genetic model herbivore that feeds on Arabidopsis will enable the parallel dissection of host defence and reciprocal resistance pathways such as those involved in xenobiotic metabolism. An ideal candidate is Scaptomyza flava, a drosophilid fly whose leafmining larvae are true herbivores that can be found in nature feeding on Arabidopsis and other crucifers. Here, we describe the life cycle of S. flava on Arabidopsis and use multiple approaches to characterize the response of Arabidopsis to S. flava attack. Oviposition choice tests and growth performance assays on different Arabidopsis ecotypes, defence-related mutants, and hormone and chitin-treated plants revealed significant differences in host preference and variation in larval performance across Arabidopsis accessions. The jasmonate and glucosinolate pathways in Arabidopsis are important in mediating quantitative resistance against S. flava, and priming with jasmonate or chitin resulted in increased resistance. Expression of xenobiotic detoxification genes was reduced in S. flava larvae reared on Arabidopsis jasmonate signalling mutants and increased in plants pretreated with chitin. These results and future research directions are discussed in the context of developing a genetic model system to analyse insect-plant interactions.

Original languageEnglish (US)
Pages (from-to)995-1014
Number of pages20
JournalMolecular Ecology
Volume20
Issue number5
DOIs
StatePublished - Mar 2011

Fingerprint

Herbivory
Arabidopsis
Drosophila
chitin
herbivore
herbivores
dissection
xenobiotics
Chitin
plant-insect interaction
larva
host preference
multiple use
ecotype
detoxification
virulence
oviposition
Genetic Models
hormone
Xenobiotics

Keywords

  • Arabidopsis
  • Drosophila
  • gene expression
  • herbivory
  • jasmonate
  • model system

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Whiteman, N. K., Groen, S. C., Chevasco, D., Bear, A., Beckwith, N., Gregory, T. R., ... Pierce, N. E. (2011). Mining the plant-herbivore interface with a leafmining Drosophila of Arabidopsis. Molecular Ecology, 20(5), 995-1014. https://doi.org/10.1111/j.1365-294X.2010.04901.x

Mining the plant-herbivore interface with a leafmining Drosophila of Arabidopsis. / Whiteman, Noah K; Groen, Simon C.; Chevasco, Daniela; Bear, Ashley; Beckwith, Noor; Gregory, T. Ryan; Denoux, Carine; Mammarella, Nicole; Ausubel, Frederick M.; Pierce, Naomi E.

In: Molecular Ecology, Vol. 20, No. 5, 03.2011, p. 995-1014.

Research output: Contribution to journalArticle

Whiteman, NK, Groen, SC, Chevasco, D, Bear, A, Beckwith, N, Gregory, TR, Denoux, C, Mammarella, N, Ausubel, FM & Pierce, NE 2011, 'Mining the plant-herbivore interface with a leafmining Drosophila of Arabidopsis', Molecular Ecology, vol. 20, no. 5, pp. 995-1014. https://doi.org/10.1111/j.1365-294X.2010.04901.x
Whiteman, Noah K ; Groen, Simon C. ; Chevasco, Daniela ; Bear, Ashley ; Beckwith, Noor ; Gregory, T. Ryan ; Denoux, Carine ; Mammarella, Nicole ; Ausubel, Frederick M. ; Pierce, Naomi E. / Mining the plant-herbivore interface with a leafmining Drosophila of Arabidopsis. In: Molecular Ecology. 2011 ; Vol. 20, No. 5. pp. 995-1014.
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