Mitogen-activated protein kinases contribute to reactive oxygen species-induced cell death in renal proximal tubule epithelial cells

Sampath Ramachandiran, Qihong Huang, Jing Dong, Serrine Lau, Terrence Monks

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Extracellular signal-regulated kinases (ERK1/2), c-Jun N-terminal kinases (JNK/SAPK), and p38 mitogen-activated protein kinase (MAPK) were all rapidly activated in a ROS-dependent manner during 2,3,5-tris-(glutathion-S-yl)hydroquinone (TGHQ)-mediated oxidative stress and oncotic cell death in renal proximal tubule epithelial cells (LLC-PK1). TGHQ-induced phosphorylation of ERK1/2 and JNK MAPKs required epidermal growth factor receptor (EGFR) activation, whereas p38 MAPK activation was EGFR independent. In contrast to their established roles in cell survival, TGHQ-activated ERK1/2 and p38 MAPK (but not JNK) appear to contribute to cell death, since inhibition of ERK1/2 or p38 MAPKs with PD098059 or SB202190 respectively, attenuated TGHQ-mediated cell death. TGHQ increased AP-1 and NFκB DNA-binding activity, but whereas pharmacological inhibition of ERK1/2 or p38 MAPKs attenuated AP-1 DNA binding activity, it potentiated TGHQ-mediated NFκB activation. Consistent with a role for NFKB activation in the cytoprotective response to ROS in renal epithelial cells, an anti-NFκB peptide SN50 suppressed the protective effects of ERK inhibition (PD098059 treatment). The data provide evidence that the activation of MAPKs by ROS in renal epithelial cells plays an important role in oncotic cell death, and NF-kB is involved in the cytoprotective effects of PD098059.

Original languageEnglish (US)
Pages (from-to)1635-1642
Number of pages8
JournalChemical Research in Toxicology
Volume15
Issue number12
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Proximal Kidney Tubule
p38 Mitogen-Activated Protein Kinases
Cell death
Mitogen-Activated Protein Kinases
Reactive Oxygen Species
Cell Death
Epithelial Cells
Chemical activation
Transcription Factor AP-1
Epidermal Growth Factor Receptor
Mitogen-Activated Protein Kinase 9
Kidney
Phosphorylation
Oxidative stress
JNK Mitogen-Activated Protein Kinases
NF-kappa B
Mitogen-Activated Protein Kinase 1
DNA
Extracellular Signal-Regulated MAP Kinases
Cell Survival

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Mitogen-activated protein kinases contribute to reactive oxygen species-induced cell death in renal proximal tubule epithelial cells. / Ramachandiran, Sampath; Huang, Qihong; Dong, Jing; Lau, Serrine; Monks, Terrence.

In: Chemical Research in Toxicology, Vol. 15, No. 12, 01.12.2002, p. 1635-1642.

Research output: Contribution to journalArticle

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