Modeling column regeneration effects on ion-exchange chromatography

Z. Karina López, Armando Tejeda, Rosa M. Montesinos, Ignacio Magaña, Roberto Z Guzman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of in-place regeneration on equilibrium and kinetic characteristics of the adsorption of bovine serum albumin to a DEAE-cellulose anion exchanger has been determined. Regeneration with sodium hydroxide and time of exposure showed no effect on equilibrium behavior. Breakthrough curves were measured for protein adsorption on fixed-bed columns and analyzed by a simple model to determine the relevant rate constants for the adsorption process. It was found that forward adsorption rate constant decreased exponentially with the chemical treatment exposure time. The implications of the results on the design and optimization of ion-exchange chromatographic processes are discussed.

Original languageEnglish (US)
Pages (from-to)99-107
Number of pages9
JournalJournal of Chromatography A
Volume791
Issue number1-2
DOIs
StatePublished - Dec 12 1997

Fingerprint

Ion Exchange Chromatography
Chromatography
Adsorption
Regeneration
Ion exchange
Rate constants
DEAE-Cellulose
Sodium Hydroxide
Ion Exchange
Bovine Serum Albumin
Anions
Kinetics
Proteins

Keywords

  • Adsorption isotherms
  • Albumin
  • Column regeneration
  • Computer simulation
  • Ion-exchange chromatography
  • Kinetic studies
  • Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Modeling column regeneration effects on ion-exchange chromatography. / López, Z. Karina; Tejeda, Armando; Montesinos, Rosa M.; Magaña, Ignacio; Guzman, Roberto Z.

In: Journal of Chromatography A, Vol. 791, No. 1-2, 12.12.1997, p. 99-107.

Research output: Contribution to journalArticle

López, Z. Karina ; Tejeda, Armando ; Montesinos, Rosa M. ; Magaña, Ignacio ; Guzman, Roberto Z. / Modeling column regeneration effects on ion-exchange chromatography. In: Journal of Chromatography A. 1997 ; Vol. 791, No. 1-2. pp. 99-107.
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