Models of cell division initiation in Chlamydomonas: A challenge to the consensus view

Deborah E. Shelton, Martin P. Leslie, Richard E. Michod

Research output: Research - peer-reviewArticle

Abstract

We develop and compare two models for division initiation in cells of the unicellular green alga Chlamydomonas reinhardtii, a topic that has remained controversial in spite of years of empirical work. Achieving a better understanding of C. reinhardtii cell cycle regulation is important because this species is used in studies of fundamental eukaryotic cell features and in studies of the evolution of multicellularity. C. reinhardtii proliferates asexually by multiple fission, interspersing rapid rounds of symmetric division with prolonged periods of growth. Our Model 1 reflects major elements of the current consensus view on C. reinhardtii division initiation, with cells first growing to a specific size, then waiting for a particular time prior to division initiation. In Model 2, our proposed alternative, growing cells divide when they have reached a growth-rate-dependent target size. The two models imply a number of different empirical patterns. We highlight these differences alongside published data, which currently fall short of unequivocally distinguishing these differences in predicted cell behavior. Nevertheless, several lines of evidence are suggestive of more Model 2-like behavior than Model 1-like behavior. Our specification of these models adds rigor to issues that have too often been worked out in relation to loose, verbal models and is directly relevant to future development of informative experiments.

LanguageEnglish (US)
Pages186-197
Number of pages12
JournalJournal of Theoretical Biology
Volume412
DOIs
StatePublished - Jan 7 2017

Fingerprint

Chlamydomonas
Cell Division
Model
Chlamydomonas reinhardtii
cell division
Cells
Cell
Chlamydomonas Reinhardtii
Division
Growth
cells
Chlorophyta
Eukaryotic Cells
Cell Cycle
Green Algae
Divides
Specification
Imply
Target
Dependent

Keywords

  • Cell cycle
  • Cell division control
  • Cell division timing
  • Chlamydomonas reinhardtii
  • Multiple fission
  • Palintomy
  • Regulation of cell number

ASJC Scopus subject areas

  • Statistics and Probability
  • Medicine(all)
  • Modeling and Simulation
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Cite this

Models of cell division initiation in Chlamydomonas : A challenge to the consensus view. / Shelton, Deborah E.; Leslie, Martin P.; Michod, Richard E.

In: Journal of Theoretical Biology, Vol. 412, 07.01.2017, p. 186-197.

Research output: Research - peer-reviewArticle

@article{7b8dafaafa1448de8bffba1439870bcc,
title = "Models of cell division initiation in Chlamydomonas: A challenge to the consensus view",
abstract = "We develop and compare two models for division initiation in cells of the unicellular green alga Chlamydomonas reinhardtii, a topic that has remained controversial in spite of years of empirical work. Achieving a better understanding of C. reinhardtii cell cycle regulation is important because this species is used in studies of fundamental eukaryotic cell features and in studies of the evolution of multicellularity. C. reinhardtii proliferates asexually by multiple fission, interspersing rapid rounds of symmetric division with prolonged periods of growth. Our Model 1 reflects major elements of the current consensus view on C. reinhardtii division initiation, with cells first growing to a specific size, then waiting for a particular time prior to division initiation. In Model 2, our proposed alternative, growing cells divide when they have reached a growth-rate-dependent target size. The two models imply a number of different empirical patterns. We highlight these differences alongside published data, which currently fall short of unequivocally distinguishing these differences in predicted cell behavior. Nevertheless, several lines of evidence are suggestive of more Model 2-like behavior than Model 1-like behavior. Our specification of these models adds rigor to issues that have too often been worked out in relation to loose, verbal models and is directly relevant to future development of informative experiments.",
keywords = "Cell cycle, Cell division control, Cell division timing, Chlamydomonas reinhardtii, Multiple fission, Palintomy, Regulation of cell number",
author = "Shelton, {Deborah E.} and Leslie, {Martin P.} and Michod, {Richard E.}",
year = "2017",
month = "1",
doi = "10.1016/j.jtbi.2016.10.018",
volume = "412",
pages = "186--197",
journal = "Journal of Theoretical Biology",
issn = "0022-5193",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Models of cell division initiation in Chlamydomonas

T2 - Journal of Theoretical Biology

AU - Shelton,Deborah E.

AU - Leslie,Martin P.

AU - Michod,Richard E.

PY - 2017/1/7

Y1 - 2017/1/7

N2 - We develop and compare two models for division initiation in cells of the unicellular green alga Chlamydomonas reinhardtii, a topic that has remained controversial in spite of years of empirical work. Achieving a better understanding of C. reinhardtii cell cycle regulation is important because this species is used in studies of fundamental eukaryotic cell features and in studies of the evolution of multicellularity. C. reinhardtii proliferates asexually by multiple fission, interspersing rapid rounds of symmetric division with prolonged periods of growth. Our Model 1 reflects major elements of the current consensus view on C. reinhardtii division initiation, with cells first growing to a specific size, then waiting for a particular time prior to division initiation. In Model 2, our proposed alternative, growing cells divide when they have reached a growth-rate-dependent target size. The two models imply a number of different empirical patterns. We highlight these differences alongside published data, which currently fall short of unequivocally distinguishing these differences in predicted cell behavior. Nevertheless, several lines of evidence are suggestive of more Model 2-like behavior than Model 1-like behavior. Our specification of these models adds rigor to issues that have too often been worked out in relation to loose, verbal models and is directly relevant to future development of informative experiments.

AB - We develop and compare two models for division initiation in cells of the unicellular green alga Chlamydomonas reinhardtii, a topic that has remained controversial in spite of years of empirical work. Achieving a better understanding of C. reinhardtii cell cycle regulation is important because this species is used in studies of fundamental eukaryotic cell features and in studies of the evolution of multicellularity. C. reinhardtii proliferates asexually by multiple fission, interspersing rapid rounds of symmetric division with prolonged periods of growth. Our Model 1 reflects major elements of the current consensus view on C. reinhardtii division initiation, with cells first growing to a specific size, then waiting for a particular time prior to division initiation. In Model 2, our proposed alternative, growing cells divide when they have reached a growth-rate-dependent target size. The two models imply a number of different empirical patterns. We highlight these differences alongside published data, which currently fall short of unequivocally distinguishing these differences in predicted cell behavior. Nevertheless, several lines of evidence are suggestive of more Model 2-like behavior than Model 1-like behavior. Our specification of these models adds rigor to issues that have too often been worked out in relation to loose, verbal models and is directly relevant to future development of informative experiments.

KW - Cell cycle

KW - Cell division control

KW - Cell division timing

KW - Chlamydomonas reinhardtii

KW - Multiple fission

KW - Palintomy

KW - Regulation of cell number

UR - http://www.scopus.com/inward/record.url?scp=84995475472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995475472&partnerID=8YFLogxK

U2 - 10.1016/j.jtbi.2016.10.018

DO - 10.1016/j.jtbi.2016.10.018

M3 - Article

VL - 412

SP - 186

EP - 197

JO - Journal of Theoretical Biology

JF - Journal of Theoretical Biology

SN - 0022-5193

ER -