Modulation by vanadate of the adrenergic characteristics of the iris, ileum, and vas deferens

N. A. Delamere, R. N. Williams

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The influence of vanadate upon the adrenergic response of the rabbit iris (dilation) was examined in vivo, while the effects of vanadate upon adrenergic responses of the rabbit ileum and guinea pig vas deferens were investigated in vitro. Intravenously administered vanadate (2 mg/Kg) sensitized the iris to topically administered epinephrine; a small quantity of epinephrine, that elicited no change of pupil size following topical administration to the eye in the normal rabbit, produced a marked pupillary dilation in animals treated systemically with vanadate. The response of the isolated ileum to vanadate (1-10 μg) was identical to that elicited by norepinephrine or epinephrine. Brief exposure of the ileum to vanadate, norepinephrine, or epinephrine resulted in a transient cessation of rhythmic activity and reduction of mean tension. When the ileum was superfused continuously with solutions containing vanadate (10 μg/ml; 5.4 x 10-5 M), the rhythmic activity and mean tension returned to control values within several minutes, suggesting tachyphylaxis. Exposure of the guinea pig vas deferens to vanadate did not elicit a contractile response. However, when the vas deferens was superfused continuously (>60 min) with vanadate (5.4 x 10-5 M), both the amplitude and duration of the contraction elicited by epinephrine were increased. Consistant with the above findings is the hypothesis that vanadate might inhibit the mechanisms responsible for the removal of epinephrine or norepinephrine from the site of action within a tissue.

Original languageEnglish (US)
Pages (from-to)1336-1341
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume27
Issue number9
StatePublished - 1986

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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