Modulation of CCK mRNA in cell lines in response to isoproterenol and retinoic acid

B. L. Mania-Farnell, I. W. Botros, Thomas P Davis

Research output: Contribution to journalArticle

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Abstract

Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC and the rat medullary thyroid carcinoma cell line WE 4/2. The cells were treated with the β-adrenergic agonist isoproterenol and retinoic acid, a natural derivative of vitamin A, which plays a role in cell growth and proliferation. Levels of CCK mRNA were determined after 6, 12 and 24 h drug treatments, with Northern blot analysis using human CCK riboprobes. In WE 4/2 cells no differences were observed in CCK mRNA levels, between control and isoproterenol treated cells, after 6, 12 or 24 h treatments. In SK-N-MCIXC cells isoproterenol increased CCK mRNA levels at all time points examined, the β-adrenergic antagonist propranolol blocked this effect. SK-N-MCIXC cells were also treated with actinomycin D or cycloheximide in combination with isoproterenol. Actinomycin D decreased CCK mRNA levels. Cycloheximide increased CCK mRNA levels when compared to isoproterenol acting alone. Retinoic acid did not affect CCK mRNA levels in WE 4/2 cells. In SK-N-MCIXC cells, retinoic acid consistently decreased CCK mRNA level. CCK mRNA levels in SK-N-MCIXC cells treated with retinoic acid combined with either isoproterenol or phorbol-12-myristate-13 acetate, were not significantly different from cells treated with retinoic acid alone.

Original languageEnglish (US)
Pages (from-to)221-227
Number of pages7
JournalNeuropeptides
Volume29
Issue number4
DOIs
StatePublished - 1995

Fingerprint

Cholecystokinin
Tretinoin
Isoproterenol
Cells
Modulation
Cell Line
Messenger RNA
Dactinomycin
Cycloheximide
Drug therapy
Adrenergic Agonists
Adrenergic Antagonists
Level control
Cell proliferation
Cell growth
Vitamin A
Propranolol
Northern Blotting
Rats
Acetates

ASJC Scopus subject areas

  • Endocrinology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neurology
  • Clinical Neurology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)
  • Biochemistry

Cite this

Modulation of CCK mRNA in cell lines in response to isoproterenol and retinoic acid. / Mania-Farnell, B. L.; Botros, I. W.; Davis, Thomas P.

In: Neuropeptides, Vol. 29, No. 4, 1995, p. 221-227.

Research output: Contribution to journalArticle

Mania-Farnell, B. L. ; Botros, I. W. ; Davis, Thomas P. / Modulation of CCK mRNA in cell lines in response to isoproterenol and retinoic acid. In: Neuropeptides. 1995 ; Vol. 29, No. 4. pp. 221-227.
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