Modulation of cerebral microvascular permeability by endothelial nicotinic acetylcholine receptors

Brian T. Hawkins, Richard D. Egleton, Thomas P Davis

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Nicotine increases the permeability of the blood-brain barrier in vivo. This implies a possible role for nicotinic acetylcholine receptors in the regulation of cerebral microvascular permeability. Expression of nicotinic acetylcholine receptor subunits in cerebral microvessels was investigated with immunofluorescence microscopy. Positive immunoreactivity was found for receptor subunits α3, α5, α7, and β2, but not subunits α4, β3, or β4. Blood-brain barrier permeability was assessed via in situ brain perfusion with [14C]sucrose. Nicotine increased the rate of sucrose entry into the brain from 0.3 ± 0.1 to 1.1 ± 0.2 μl·g-1·min-1, as previously described. This nicotine-induced increase in blood-brain barrier permeability was significantly attenuated by both the blood-brain barrier-permeant nicotinic antagonist mecamylamine and the blood-brain barrier-impermeant nicotinic antagonist hexamethonium to 0.5 ± 0.2 and 0.3 ± 0.2 μl·g-1·min-1, respectively. These data suggest that nicotinic acetylcholine receptors expressed on the cerebral microvascular endothelium mediate nicotine-induced changes in blood-brain barrier permeability.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume289
Issue number1 58-1
DOIs
StatePublished - Jul 2005

Fingerprint

Nicotinic Receptors
Capillary Permeability
Blood-Brain Barrier
Nicotine
Permeability
Nicotinic Antagonists
Sucrose
Mecamylamine
Hexamethonium
Brain
Microvessels
Fluorescence Microscopy
Endothelium
Perfusion

Keywords

  • Blood-barin barrier
  • Hexamethonium
  • Immunofluorescence microscopy
  • Mecamylamine
  • Nicotine

ASJC Scopus subject areas

  • Physiology

Cite this

Modulation of cerebral microvascular permeability by endothelial nicotinic acetylcholine receptors. / Hawkins, Brian T.; Egleton, Richard D.; Davis, Thomas P.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 289, No. 1 58-1, 07.2005.

Research output: Contribution to journalArticle

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