Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol

Niel C. Hoglen, Edward A. Abril, John Michael Sauer, David L. Earnest, Robert S. McCuskey, R. Clark Lantz, Scott A. Mobley, Glenn I. Sipes

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16 Scopus citations

Abstract

Previous studies have shown that large doses of vitamin A potentiate chemical-induced liver injury and that the Kupffer cell is directly involved in this potentiation. Therefore, these studies were designed to determine if Kupffer cells isolated from vitamin A treated male Sprague-Dawley rats (75 mg/kg/day for 3-7 days as all-trans-retinol) had altered activity and function. Respiratory activity of Kupffer cells isolated from rats treated with vitamin A for 3 to 7 days markedly increased. Similarly, phagocytic activity was significantly elevated (up to 9-fold) after exposure to vitamin A for 3 to 7 days. Production of reactive oxygen species, measured by luminol-enhanced chemiluminescence of Kupffer cells isolated after 7 days of vitamin A exposure, was significantly higher than that of control cells when stimulated with opsonized zymosan. Also, the release of superoxide anion by individual Kupffer cells isolated from vitamin A treated rats was nearly three times greater than that of control cells. Basal production of tumor necrosis factor-alpha (TNF-α) and prostaglandin E2 (PGE2) production were significantly elevated in Kupffer cells isolated from rats treated with vitamin A. Lastly, peripheral blood monocytes (PBMC) isolated from rats treated with vitamin A for 7 days had a significantly greater respiratory activity, as well as TNF-α and PGE2 production, than PBMC isolated from control rats. Our data suggest that large doses of vitamin A enhance both Kupffer cell and PBMC function. Upregulation of the activity by these phagocytic cells may play a role in the vitamin A potentiation of chemical-induced liver injury.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalLiver
Volume17
Issue number3
StatePublished - Jun 1 1997

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Keywords

  • Liver injury
  • Macrophage
  • Reactive oxygen species
  • TNF
  • Vitamin A

ASJC Scopus subject areas

  • Hepatology

Cite this

Hoglen, N. C., Abril, E. A., Sauer, J. M., Earnest, D. L., McCuskey, R. S., Lantz, R. C., Mobley, S. A., & Sipes, G. I. (1997). Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol. Liver, 17(3), 157-165.