Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol

Niel C. Hoglen, Edward A. Abril, John Michael Sauer, David L. Earnest, Robert S. McCuskey, Robert Clark Lantz, Scott A. Mobley, Glenn I. Sipes

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Previous studies have shown that large doses of vitamin A potentiate chemical-induced liver injury and that the Kupffer cell is directly involved in this potentiation. Therefore, these studies were designed to determine if Kupffer cells isolated from vitamin A treated male Sprague-Dawley rats (75 mg/kg/day for 3-7 days as all-trans-retinol) had altered activity and function. Respiratory activity of Kupffer cells isolated from rats treated with vitamin A for 3 to 7 days markedly increased. Similarly, phagocytic activity was significantly elevated (up to 9-fold) after exposure to vitamin A for 3 to 7 days. Production of reactive oxygen species, measured by luminol-enhanced chemiluminescence of Kupffer cells isolated after 7 days of vitamin A exposure, was significantly higher than that of control cells when stimulated with opsonized zymosan. Also, the release of superoxide anion by individual Kupffer cells isolated from vitamin A treated rats was nearly three times greater than that of control cells. Basal production of tumor necrosis factor-alpha (TNF-α) and prostaglandin E2 (PGE2) production were significantly elevated in Kupffer cells isolated from rats treated with vitamin A. Lastly, peripheral blood monocytes (PBMC) isolated from rats treated with vitamin A for 7 days had a significantly greater respiratory activity, as well as TNF-α and PGE2 production, than PBMC isolated from control rats. Our data suggest that large doses of vitamin A enhance both Kupffer cell and PBMC function. Upregulation of the activity by these phagocytic cells may play a role in the vitamin A potentiation of chemical-induced liver injury.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalLiver
Volume17
Issue number3
StatePublished - Jun 1997

Fingerprint

Kupffer Cells
Vitamin A
Monocytes
Dinoprostone
Tumor Necrosis Factor-alpha
Luminol
Zymosan
Liver
Wounds and Injuries
Phagocytes
Luminescence
Superoxides
Sprague Dawley Rats
Reactive Oxygen Species
Up-Regulation

Keywords

  • Liver injury
  • Macrophage
  • Reactive oxygen species
  • TNF
  • Vitamin A

ASJC Scopus subject areas

  • Hepatology

Cite this

Hoglen, N. C., Abril, E. A., Sauer, J. M., Earnest, D. L., McCuskey, R. S., Lantz, R. C., ... Sipes, G. I. (1997). Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol. Liver, 17(3), 157-165.

Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol. / Hoglen, Niel C.; Abril, Edward A.; Sauer, John Michael; Earnest, David L.; McCuskey, Robert S.; Lantz, Robert Clark; Mobley, Scott A.; Sipes, Glenn I.

In: Liver, Vol. 17, No. 3, 06.1997, p. 157-165.

Research output: Contribution to journalArticle

Hoglen, NC, Abril, EA, Sauer, JM, Earnest, DL, McCuskey, RS, Lantz, RC, Mobley, SA & Sipes, GI 1997, 'Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol', Liver, vol. 17, no. 3, pp. 157-165.
Hoglen NC, Abril EA, Sauer JM, Earnest DL, McCuskey RS, Lantz RC et al. Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol. Liver. 1997 Jun;17(3):157-165.
Hoglen, Niel C. ; Abril, Edward A. ; Sauer, John Michael ; Earnest, David L. ; McCuskey, Robert S. ; Lantz, Robert Clark ; Mobley, Scott A. ; Sipes, Glenn I. / Modulation of Kupffer cell and peripheral blood monocyte activity by in vivo treatment of rats with all-trans-retinol. In: Liver. 1997 ; Vol. 17, No. 3. pp. 157-165.
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abstract = "Previous studies have shown that large doses of vitamin A potentiate chemical-induced liver injury and that the Kupffer cell is directly involved in this potentiation. Therefore, these studies were designed to determine if Kupffer cells isolated from vitamin A treated male Sprague-Dawley rats (75 mg/kg/day for 3-7 days as all-trans-retinol) had altered activity and function. Respiratory activity of Kupffer cells isolated from rats treated with vitamin A for 3 to 7 days markedly increased. Similarly, phagocytic activity was significantly elevated (up to 9-fold) after exposure to vitamin A for 3 to 7 days. Production of reactive oxygen species, measured by luminol-enhanced chemiluminescence of Kupffer cells isolated after 7 days of vitamin A exposure, was significantly higher than that of control cells when stimulated with opsonized zymosan. Also, the release of superoxide anion by individual Kupffer cells isolated from vitamin A treated rats was nearly three times greater than that of control cells. Basal production of tumor necrosis factor-alpha (TNF-α) and prostaglandin E2 (PGE2) production were significantly elevated in Kupffer cells isolated from rats treated with vitamin A. Lastly, peripheral blood monocytes (PBMC) isolated from rats treated with vitamin A for 7 days had a significantly greater respiratory activity, as well as TNF-α and PGE2 production, than PBMC isolated from control rats. Our data suggest that large doses of vitamin A enhance both Kupffer cell and PBMC function. Upregulation of the activity by these phagocytic cells may play a role in the vitamin A potentiation of chemical-induced liver injury.",
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