Modulation of potassium transport in cultured retinal pigment epithelium and retinal glial cells by serum and epidermal growth factor

Everton L. Arrindell, Brian S Mckay, Glenn J. Jaffe, Janice M. Burke

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The ionic environment of retinal photoreceptors is partially controlled by potassium transporters on retinal glial and retinal pigment epithelial cells (RPE). In this study, serum and epidermal growth factor (EGF) were examined as modulators of potassium transport in confluent cultures of human RPE and rabbit retinal glia. EGF is a known mitogen for confluent RPE cultures and was shown here to also stimulate [3H]-thymidine incorporation in cultures of retinal glia. For potassium transport studies 86Rb was used as a tracer during a 17-min incubation. For both retinal cell types the mean total 88Rb uptake in 10% serum was approximately 60% above basal, serum-free controls. For EGF, tested in several experiments in a concentration range from 1 to 100 ng/ml, maximal total uptake was 33 and 24% above controls for RPE and glia, respectively. Inhibitor studies suggested that basal and serum-stimulated uptake for both cell types occurred by the ouabain-sensitive Na-K ATPase pump and by the furosemide- or bumetanide-sensitive Na-K-Cl cotransporter. EGF-stimulated uptake appeared to be due predominantly to the cotransporter. The data suggest that serum components and EGF, which may be available to retina-derived cells under pathologic conditions, may not only stimulate proliferation but may also act as short-term modulators of potassium ion movement and thus affect physiologic processes that are sensitive to ion homeostasis.

Original languageEnglish (US)
Pages (from-to)192-197
Number of pages6
JournalExperimental Cell Research
Volume203
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Retinal Pigment Epithelium
Epidermal Growth Factor
Neuroglia
Retinal Pigments
Potassium
Epithelial Cells
Serum
Sodium-Potassium-Chloride Symporters
Ions
Bumetanide
Vertebrate Photoreceptor Cells
Furosemide
Ouabain
Mitogens
Thymidine
Retina
Homeostasis
Cell Culture Techniques
Rabbits

ASJC Scopus subject areas

  • Cell Biology

Cite this

Modulation of potassium transport in cultured retinal pigment epithelium and retinal glial cells by serum and epidermal growth factor. / Arrindell, Everton L.; Mckay, Brian S; Jaffe, Glenn J.; Burke, Janice M.

In: Experimental Cell Research, Vol. 203, No. 1, 1992, p. 192-197.

Research output: Contribution to journalArticle

@article{952317f342484421b7b789215f9bce2b,
title = "Modulation of potassium transport in cultured retinal pigment epithelium and retinal glial cells by serum and epidermal growth factor",
abstract = "The ionic environment of retinal photoreceptors is partially controlled by potassium transporters on retinal glial and retinal pigment epithelial cells (RPE). In this study, serum and epidermal growth factor (EGF) were examined as modulators of potassium transport in confluent cultures of human RPE and rabbit retinal glia. EGF is a known mitogen for confluent RPE cultures and was shown here to also stimulate [3H]-thymidine incorporation in cultures of retinal glia. For potassium transport studies 86Rb was used as a tracer during a 17-min incubation. For both retinal cell types the mean total 88Rb uptake in 10{\%} serum was approximately 60{\%} above basal, serum-free controls. For EGF, tested in several experiments in a concentration range from 1 to 100 ng/ml, maximal total uptake was 33 and 24{\%} above controls for RPE and glia, respectively. Inhibitor studies suggested that basal and serum-stimulated uptake for both cell types occurred by the ouabain-sensitive Na-K ATPase pump and by the furosemide- or bumetanide-sensitive Na-K-Cl cotransporter. EGF-stimulated uptake appeared to be due predominantly to the cotransporter. The data suggest that serum components and EGF, which may be available to retina-derived cells under pathologic conditions, may not only stimulate proliferation but may also act as short-term modulators of potassium ion movement and thus affect physiologic processes that are sensitive to ion homeostasis.",
author = "Arrindell, {Everton L.} and Mckay, {Brian S} and Jaffe, {Glenn J.} and Burke, {Janice M.}",
year = "1992",
doi = "10.1016/0014-4827(92)90055-D",
language = "English (US)",
volume = "203",
pages = "192--197",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Modulation of potassium transport in cultured retinal pigment epithelium and retinal glial cells by serum and epidermal growth factor

AU - Arrindell, Everton L.

AU - Mckay, Brian S

AU - Jaffe, Glenn J.

AU - Burke, Janice M.

PY - 1992

Y1 - 1992

N2 - The ionic environment of retinal photoreceptors is partially controlled by potassium transporters on retinal glial and retinal pigment epithelial cells (RPE). In this study, serum and epidermal growth factor (EGF) were examined as modulators of potassium transport in confluent cultures of human RPE and rabbit retinal glia. EGF is a known mitogen for confluent RPE cultures and was shown here to also stimulate [3H]-thymidine incorporation in cultures of retinal glia. For potassium transport studies 86Rb was used as a tracer during a 17-min incubation. For both retinal cell types the mean total 88Rb uptake in 10% serum was approximately 60% above basal, serum-free controls. For EGF, tested in several experiments in a concentration range from 1 to 100 ng/ml, maximal total uptake was 33 and 24% above controls for RPE and glia, respectively. Inhibitor studies suggested that basal and serum-stimulated uptake for both cell types occurred by the ouabain-sensitive Na-K ATPase pump and by the furosemide- or bumetanide-sensitive Na-K-Cl cotransporter. EGF-stimulated uptake appeared to be due predominantly to the cotransporter. The data suggest that serum components and EGF, which may be available to retina-derived cells under pathologic conditions, may not only stimulate proliferation but may also act as short-term modulators of potassium ion movement and thus affect physiologic processes that are sensitive to ion homeostasis.

AB - The ionic environment of retinal photoreceptors is partially controlled by potassium transporters on retinal glial and retinal pigment epithelial cells (RPE). In this study, serum and epidermal growth factor (EGF) were examined as modulators of potassium transport in confluent cultures of human RPE and rabbit retinal glia. EGF is a known mitogen for confluent RPE cultures and was shown here to also stimulate [3H]-thymidine incorporation in cultures of retinal glia. For potassium transport studies 86Rb was used as a tracer during a 17-min incubation. For both retinal cell types the mean total 88Rb uptake in 10% serum was approximately 60% above basal, serum-free controls. For EGF, tested in several experiments in a concentration range from 1 to 100 ng/ml, maximal total uptake was 33 and 24% above controls for RPE and glia, respectively. Inhibitor studies suggested that basal and serum-stimulated uptake for both cell types occurred by the ouabain-sensitive Na-K ATPase pump and by the furosemide- or bumetanide-sensitive Na-K-Cl cotransporter. EGF-stimulated uptake appeared to be due predominantly to the cotransporter. The data suggest that serum components and EGF, which may be available to retina-derived cells under pathologic conditions, may not only stimulate proliferation but may also act as short-term modulators of potassium ion movement and thus affect physiologic processes that are sensitive to ion homeostasis.

UR - http://www.scopus.com/inward/record.url?scp=0026448502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026448502&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(92)90055-D

DO - 10.1016/0014-4827(92)90055-D

M3 - Article

C2 - 1330655

AN - SCOPUS:0026448502

VL - 203

SP - 192

EP - 197

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -