Modulation of pulmonary alveolar type II cell phenotype and communication by extracellular matrix and KGF

Brant E. Isakson, Richard L. Lubman, Gregory J. Seedorf, Scott Boitano

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

The alveolar epithelium consists of two cell types, alveolar type I (AT1) and alveolar type II (AT2) cells. We have recently shown that 7-day-old cultures of AT2 cells grown on a type I collagen/fibronectin matrix develop phenotypic characteristics of AT1 cells, display a distinct connexin profile, and coordinate mechanically induced intercellular Ca2+ changes via gap junctions (25). In this study, we cultured AT2 cells for 7 days on matrix supplemented with laminin-5 and/or in the presence of keratinocyte growth factor. Under these conditions, cultured AT2 cells display AT2 type morphology, express the AT2-specific marker surfactant protein C, and do not express AT1-specific cell marker aquaporin 5, all consistent with maintenance of AT2 phenotype. These AT2-like cells also coordinate mechanically induced intercellular Ca2+ signaling, but, unlike AT1-like cells, do so by using extracellular nucleotide triphosphate release. Additionally, cultured cells that retain AT2 cell-specific markers express connexin profiles different from cultured cells with AT1 characteristics. The parallel changes in intercellular Ca2+ signaling with cell differentiation suggest that cell signaling mechanisms are an intrinsic component of lung alveolar cell phenotype. Because lung epithelial injury is accompanied by extracellular matrix and growth factor changes, followed by extensive cell division, differentiation, and migration of AT2 progenitor cells, we suggest that similar changes may be vital to the lung recovery and repair process in vivo.

Original languageEnglish (US)
Pages (from-to)C1291-C1299
JournalAmerican Journal of Physiology - Cell Physiology
Volume281
Issue number4 50-4
StatePublished - Oct 20 2001
Externally publishedYes

Keywords

  • Connexin
  • Gap junction
  • Intercellular communication
  • Keratinocyte growth factor
  • Laminin-5

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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