Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts: p53 and Rb control G1 arrest but not cell replication

Qin Chen, James C. Bartholomew, Judith Campisi, Meileen Acosta, Joshua D. Reagan, Bruce N. Ames

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Abstract

Human diploid fibroblasts lose the capacity to proliferate and enter a state termed replicative senescence after a finite number of cell divisions in culture. When treated with sub-lethal concentrations of H2O2, pre-senescent human fibroblasts enter long-term growth arrest resembling replicative senescence. To understand the molecular basis for the H2O2-induced growth arrest, we determined the cell cycle distribution, levels of p53 tumour suppressor and p21 cyclin-dependent kinase inhibitor proteins, and the status of Rb phosphorylation in H2O2-treated cells. A 2 h pulse of H2O2 arrested the growth of IMR-90 fetal lung fibroblasts for at least 15 days. The arrested cells showed a G1 DNA content. The level of p53 protein increased 2- to 3-fold within 1.5 h after H2O2 exposure but returned to the control level by 48 h. The induction of p53 protein was dose dependent, beginning at 50-75 μM and reaching a maximum at 100-250 μM. The induction of p53 did not appear to correlate with the level of DNA damage as measured by the formation of 8-oxo-2'-deoxyguanosine in DNA. The level of p21 protein increased about 18 h after H2O2 exposure and remained elevated for at least 21 days. During this period, Rb remained under-phosphorylated. The induction of p53 by H2O2 was abolished by the iron chelator deferoxamine and the protein synthesis inhibitor cycloheximide. The human papillomavirus protein E6, when introduced into the cells, abolished the induction of p53, reduced the induction of p21 to a minimal level and allowed Rb phosphorylation and entry of the cells into S-phase. The human papillomavirus protein E7 reduced the overall level of Rb and also abolished H2O2-induced G1 arrest. Inactivating G1 arrest by E6, E7 or both did not restore the replicative ability of H2O2-treated cells. Thus H2O2-treated cells show a transient elevation of p53, high level of p21, lack of Rb phosphorylation, G1 arrest and inability to replicate when G1 arrest is inactivated.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalBiochemical Journal
Volume332
Issue number1
StatePublished - May 15 1998

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Fibroblasts
Cells
Phosphorylation
Growth
Cell Aging
Papillomavirus E7 Proteins
DNA
Proteins
Cyclin-Dependent Kinase Inhibitor Proteins
Protein Synthesis Inhibitors
Deferoxamine
Level control
Cycloheximide
Chelating Agents
Cell culture
Tumors
Cell Cycle Checkpoints
Diploidy
S Phase
Cell Division

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts : p53 and Rb control G1 arrest but not cell replication. / Chen, Qin; Bartholomew, James C.; Campisi, Judith; Acosta, Meileen; Reagan, Joshua D.; Ames, Bruce N.

In: Biochemical Journal, Vol. 332, No. 1, 15.05.1998, p. 43-50.

Research output: Contribution to journalArticle

Chen, Qin ; Bartholomew, James C. ; Campisi, Judith ; Acosta, Meileen ; Reagan, Joshua D. ; Ames, Bruce N. / Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts : p53 and Rb control G1 arrest but not cell replication. In: Biochemical Journal. 1998 ; Vol. 332, No. 1. pp. 43-50.
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