Molecular analysis of the induction of immunoglobulin E synthesis in human B cells by interleukin 4 and engagement of CD40 antigen

Stuart K. Shapira, Donata Vercelli, Haifa H. Jabara, Shu Man Fu, Raif S. Geha

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The molecular events leading to immunoglobulin E (IgE) synthesis in human sIgE- B cells stimulated with interleukin 4 (IL-4) and anti-CD40 monoclonal antibody (mAb) 626.1 were analyzed. Anti-CD40 mAb increased the levels of IL-4-induced germline C∈ transcripts and induced the production of mature C∈ mRNA. These effects were dependent on the presence of IL-4. Nested primer PCR revealed deletional switch recombination occurring only in B cells stimulated with both IL-4 and anti-CD40 mAb. DNA sequence analysis of switch fragments showed direct SμS∈ joining, without the deletions or duplications within Sμ often found in B cells stimulated with IL-4 and Epstein-Barr virus. Analysis of the switch junction map sites showed "hot spots" for recombination within Sμ, but not within S∈. These findings indicate that IL-4 provides a signal to B cells to induce germline C∈ transcription and concurrent CD40 engagement induces Sμ/S∈ deletional switch recombination, production of mature C∈ mRNA, and IgE synthesis.

Original languageEnglish (US)
Pages (from-to)289-292
Number of pages4
JournalJournal of Experimental Medicine
Volume175
Issue number1
StatePublished - 1992
Externally publishedYes

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CD40 Antigens
Interleukin-4
Immunoglobulin E
B-Lymphocytes
Genetic Recombination
Monoclonal Antibodies
Messenger RNA
Human Herpesvirus 4
DNA Sequence Analysis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular analysis of the induction of immunoglobulin E synthesis in human B cells by interleukin 4 and engagement of CD40 antigen. / Shapira, Stuart K.; Vercelli, Donata; Jabara, Haifa H.; Fu, Shu Man; Geha, Raif S.

In: Journal of Experimental Medicine, Vol. 175, No. 1, 1992, p. 289-292.

Research output: Contribution to journalArticle

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