Molecular analysis of the serine/threonine kinase Akt and its expression in the mosquito Aedes aegypti

M. A. Riehle, M. R. Brown

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A key component of the insulin-signalling pathway, the protein kinase Akt, was identified and cloned as a cDNA from ovaries of the mosquito Aedes aegypti. An ortholog gene was found in the Anopheles gambiae genome database, and like other Akts, both mosquito Akts possess pleckstrin homology domains for membrane binding and a serine/threonine kinase domain. When Ae. aegypti ovaries were treated with bovine insulin in vitro, a putative Akt was threonine-phosphorylated, as expected for Akts. AaegAKT was only expressed in embryos for the first 6 h after oviposition and in ovaries before and during a gonotrophic cycle.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalInsect Molecular Biology
Volume12
Issue number3
DOIs
StatePublished - Jun 2003
Externally publishedYes

Keywords

  • Ecdysteroids
  • Insect
  • Oogenesis
  • Ovary
  • Protein kinase B (PKB)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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