Molecular and biological characterization of a novel mild strain of citrus tristeza virus in California

Raymond Yokomi, Vijayanandraj Selvaraj, Yogita Maheshwari, Michela Chiumenti, Maria Saponari, Annalisa Giampetruzzi, Ziming Weng, Zhongguo Xiong, Subhas Hajeri

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Strain differentiating marker profiles of citrus tristeza virus (CTV) isolates from California have shown the presence of multiple genotypes. To better define the genetic diversity involved, full-length genome sequences from four California CTV isolates were determined by small-interfering RNA sequencing. Phylogenetic analysis and nucleotide sequence comparisons differentiated these isolates into the genotypes VT (CA-VT-AT39), T30 (CA-T30-AT4), and a new strain called S1 (CA-S1-L and CA-S1-L65). S1 isolates had three common recombination events within portions of genes from VT, T36 and RB strains and were transmissible by Aphis gossypii. Virus indexing showed that CA-VT-AT39 could be classified as a severe strain, whereas CA-T30-AT4, CA-S1-L and CA-S1-L65 were mild. CA-VT-AT39, CA-S1-L, and CA-S1-L65 reacted with monoclonal antibody MCA13, whereas CA-T30-AT4 did not. RT-PCR and RT-qPCR detection assays for the S1 strain were developed and used to screen MCA13-reactive isolates in a CTV collection from central California collected from 1968 to 2011. Forty-two isolates were found to contain the S1 strain, alone or in combinations with other genotypes. BLAST and phylogenetic analysis of the S1 p25 gene region with other extant CTV sequences from the NCBI database suggested that putative S1-like isolates might occur elsewhere (e.g., China, South Korea, Turkey, Bosnia and Croatia). This information is important for CTV evolution, detection of specific strains, and cross-protection.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalArchives of Virology
DOIs
StateAccepted/In press - Mar 17 2018

Fingerprint

California Encephalitis Viruses
Citrus
Viruses
Genotype
Bosnia and Herzegovina
Cross Protection
RNA Sequence Analysis
Republic of Korea
Croatia
Turkey
Small Interfering RNA
Genetic Recombination
Genes
China
Monoclonal Antibodies
Genome
Databases
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology

Cite this

Yokomi, R., Selvaraj, V., Maheshwari, Y., Chiumenti, M., Saponari, M., Giampetruzzi, A., ... Hajeri, S. (Accepted/In press). Molecular and biological characterization of a novel mild strain of citrus tristeza virus in California. Archives of Virology, 1-10. https://doi.org/10.1007/s00705-018-3799-5

Molecular and biological characterization of a novel mild strain of citrus tristeza virus in California. / Yokomi, Raymond; Selvaraj, Vijayanandraj; Maheshwari, Yogita; Chiumenti, Michela; Saponari, Maria; Giampetruzzi, Annalisa; Weng, Ziming; Xiong, Zhongguo; Hajeri, Subhas.

In: Archives of Virology, 17.03.2018, p. 1-10.

Research output: Contribution to journalArticle

Yokomi, R, Selvaraj, V, Maheshwari, Y, Chiumenti, M, Saponari, M, Giampetruzzi, A, Weng, Z, Xiong, Z & Hajeri, S 2018, 'Molecular and biological characterization of a novel mild strain of citrus tristeza virus in California', Archives of Virology, pp. 1-10. https://doi.org/10.1007/s00705-018-3799-5
Yokomi, Raymond ; Selvaraj, Vijayanandraj ; Maheshwari, Yogita ; Chiumenti, Michela ; Saponari, Maria ; Giampetruzzi, Annalisa ; Weng, Ziming ; Xiong, Zhongguo ; Hajeri, Subhas. / Molecular and biological characterization of a novel mild strain of citrus tristeza virus in California. In: Archives of Virology. 2018 ; pp. 1-10.
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