Molecular and Cytogenetic Characterization of a Chinese Hamster/Human Hybrid Cell Line Containing a der (21)t(Ypter→cenY::cen21 → 21qter) Chromosome

David Patterson, Iris Hart, Li Wen Lai, Christina Brahe, Alessandra Moscetti, Flora Tassone, Elena Raimondi, Carol Jones

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Abstract

Human/rodent somatic cell hybrids have been exceedingly useful in assigning human genes and DNA sequences to specific human chromosomes. As new technologies for analyzing the human chromosome complement of such human/rodent hybrid cells become available, it is of critical importance that these be applied to enhance characterization of existing hybrids. This is particularly important since human chromosomes in such hybrids have been observed to rearrange with time. We report here the use of fluorescence in situ hybridization of DNA probes to metaphase chromosomes to analyze one hybrid designated 72532X6. This analysis shows that the chromosome suspected to be a normal human chromosome 21 in this hybrid is actually a translocation chromosome containing Yp and 21q. In addition, the hybrid contains a fragment of human chromosome 9 translocated to a Chinese hamster chromosome. Analysis of the chromosomes from the human donor indicates that his chromosomes are normal. Thus, this translocatlon chromosome appears to have arisen after formation of the hybrid.

Original languageEnglish (US)
Pages (from-to)177-179
Number of pages3
JournalGenomics
Volume15
Issue number1
DOIs
StatePublished - Jan 1993

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ASJC Scopus subject areas

  • Genetics

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