Molecular characterization and differential expression of olfactory genes in the antennae of the black cutworm moth Agrotis ipsilon

Shao Hua Gu, Liang Sun, Ruo Nan Yang, Kong Ming Wu, Yu Yuan Guo, Xianchun Li, Jing Jiang Zhou, Yong Jun Zhang

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Insects use their sensitive and selective olfactory system to detect outside chemical odorants, such as female sex pheromones and host plant volatiles. Several groups of olfactory proteins participate in the odorant detection process, including odorant binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs) and sensory neuron membrane proteins (SNMPs). The identification and functional characterization of these olfactory proteins will enhance our knowledge of the molecular basis of insect chemoreception. In this study, we report the identification and differential expression profiles of these olfactory genes in the black cutworm moth Agrotis ipsilon. In total, 33 OBPs, 12 CSPs, 42 ORs, 24 IRs, 2 SNMPs and 1 gustatory receptor (GR) were annotated from the A. ipsilon antennal transcriptomes, and further RT-PCR and RT-qPCR revealed that 22 OBPs, 3 CSPs, 35 ORs, 14 IRs and the 2 SNMPs are uniquely or primarily expressed in the male and female antennae. Furthermore, one OBP (AipsOBP6) and one CSP (AipsCSP2) were exclusively expressed in the female sex pheromone gland. These antennae-enriched OBPs, CSPs, ORs, IRs and SNMPs were suggested to be responsible for pheromone and general odorant detection and thus could be meaningful target genes for us to study their biological functions in vivo and in vitro.

Original languageEnglish (US)
Article number0103420
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 1 2014
Externally publishedYes

Fingerprint

Agrotis ipsilon
Odorant Receptors
Moths
antennae
moths
Genes
Antennas
Gene Expression
Sensory Receptor Cells
Neurons
Sex Attractants
sensory neurons
Membrane Proteins
membrane proteins
Proteins
Transcriptome
genes
Insects
odor compounds
receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Molecular characterization and differential expression of olfactory genes in the antennae of the black cutworm moth Agrotis ipsilon. / Gu, Shao Hua; Sun, Liang; Yang, Ruo Nan; Wu, Kong Ming; Guo, Yu Yuan; Li, Xianchun; Zhou, Jing Jiang; Zhang, Yong Jun.

In: PLoS One, Vol. 9, No. 8, 0103420, 01.08.2014.

Research output: Contribution to journalArticle

Gu, Shao Hua ; Sun, Liang ; Yang, Ruo Nan ; Wu, Kong Ming ; Guo, Yu Yuan ; Li, Xianchun ; Zhou, Jing Jiang ; Zhang, Yong Jun. / Molecular characterization and differential expression of olfactory genes in the antennae of the black cutworm moth Agrotis ipsilon. In: PLoS One. 2014 ; Vol. 9, No. 8.
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