Molecular characterization and in situ quantification of anoxic arsenite-oxidizing denitrifying enrichment cultures

Wenjie Sun, Maria Reye Sierra Alvarez, Nuria Fernandez, Jose Luis Sanz, Ricardo Amils, Antje Legatzki, Raina Margaret Maier, James A Field

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

To explore the bacteria involved in the oxidation of arsenite (As(III)) under denitrifying conditions, three enrichment cultures (ECs) and one mixed culture (MC) were characterized that originated from anaerobic environmental samples. The oxidation of As(III) (0.5 mM) was dependent on NO3 - addition and N2 formation was dependent on As(III) addition. The ratio of N2-N formed to As(III) fed approximated the expected stoichiometry of 2.5. A 16S rRNA gene clone library analysis revealed three predominant phylotypes. The first, related to the genus Azoarcus from the division Betaproteobacteria, was found in the three ECs. The other two predominant phylotypes were closely related to the genera Acidovorax and Diaphorobacter within the Comamonadaceae family of Betaproteobacteria, and one of these was present in all of the cultures examined. FISH confirmed that Azoarcus accounted for a large fraction of bacteria present in the ECs. The Azoarcus clones had 96% sequence homology with Azoarcus sp. strain DAO1, an isolate previously reported to oxidize As(III) with nitrate. FISH analysis also confirmed that Comamonadaceae were present in all cultures. Pure cultures of Azoarcus and Diaphorobacter were isolated and shown to be responsible for nitrate-dependent As(III) oxidation. These results, taken as a whole, suggest that bacteria within the genus Azoarcus and the family Comamonadaceae are involved in the observed anoxic oxidation of As(III).

Original languageEnglish (US)
Pages (from-to)72-85
Number of pages14
JournalFEMS Microbiology Ecology
Volume68
Issue number1
DOIs
StatePublished - Apr 2009

Fingerprint

Azoarcus
arsenite
Comamonadaceae
Betaproteobacteria
oxidation
Bacteria
Nitrates
bacterium
Clone Cells
clone
nitrate
Sequence Homology
Gene Library
rRNA Genes
stoichiometry
in situ
homology

Keywords

  • Anoxic
  • Arsenite
  • Azoarcus
  • Chemolithotrophic
  • Comamonadaceae
  • Oxidation

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Molecular characterization and in situ quantification of anoxic arsenite-oxidizing denitrifying enrichment cultures. / Sun, Wenjie; Sierra Alvarez, Maria Reye; Fernandez, Nuria; Sanz, Jose Luis; Amils, Ricardo; Legatzki, Antje; Maier, Raina Margaret; Field, James A.

In: FEMS Microbiology Ecology, Vol. 68, No. 1, 04.2009, p. 72-85.

Research output: Contribution to journalArticle

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