Molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in Yucatan, Mexico

Cecilia Hernández-Zepeda, Ali M. Idris, Germán Carnevali, Judith K Brown, Oscar A. Moreno-Valenzuela

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Sida acuta and Corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the Yucatan Peninsula, Mexico. Total DNA was isolated from both plant species and used for the amplification, cloning, and sequencing of the Begomovirus genome. Nucleotide comparison of the complete DNA-A component isolated from S. acuta and C. siliquosus confirmed the presence of two distinct begomoviruses species. Based on phenotypic symptoms observed in infected field plants, the names Sida yellow mosaic Yucatan virus (SiYMYuV) and Corchorus yellow vein Yucatan virus (CoYVYuV) were proposed. The SiYMYuV DNA-A shared the highest nucleotide identity (86%) with the Okra yellow mosaic Mexico virus (OkYMMV). The complete DNA-B component shared the highest nucleotide identity (80%) with CoYVYuV. The CoYVYuV DNA-A shared the highest nucleotide identity (84%) with SiYMYuV. The 166-nt common region (CR) sequence for the DNA-A and DNA-B components of SiYMYuV shared a high nucleotide identity of 99%, and the 151 nt of CoYVYuV CR shared 95% of nucleotide identity. The organization and the iterated sequence of the putative AC1 binding site (located within the common region) of both isolates, were similar to that of the begomoviruses of the Western Hemisphere. Phylogenetic analyses placed the DNA-A and DNA-B of SiYMYuV and CoYVYuV in the clade containing the Abutilon mosaic virus (AbMV).

Original languageEnglish (US)
Pages (from-to)369-377
Number of pages9
JournalVirus Genes
Volume35
Issue number2
DOIs
StatePublished - Oct 2007

Fingerprint

Begomovirus
Corchorus
Mosaic Viruses
Mexico
Veins
Nucleotides
DNA
Viruses
Malvaceae
Abelmoschus
DNA Viruses
Names
Organism Cloning
Binding Sites
Genome

Keywords

  • Begomovirus
  • Geminiviridae
  • Malvaceae
  • Molecular characterization
  • ssDNA virus
  • Weed

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in Yucatan, Mexico. / Hernández-Zepeda, Cecilia; Idris, Ali M.; Carnevali, Germán; Brown, Judith K; Moreno-Valenzuela, Oscar A.

In: Virus Genes, Vol. 35, No. 2, 10.2007, p. 369-377.

Research output: Contribution to journalArticle

Hernández-Zepeda, Cecilia ; Idris, Ali M. ; Carnevali, Germán ; Brown, Judith K ; Moreno-Valenzuela, Oscar A. / Molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in Yucatan, Mexico. In: Virus Genes. 2007 ; Vol. 35, No. 2. pp. 369-377.
@article{0dcafc78057f4f5ab67b2b26984ce66f,
title = "Molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in Yucatan, Mexico",
abstract = "Sida acuta and Corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the Yucatan Peninsula, Mexico. Total DNA was isolated from both plant species and used for the amplification, cloning, and sequencing of the Begomovirus genome. Nucleotide comparison of the complete DNA-A component isolated from S. acuta and C. siliquosus confirmed the presence of two distinct begomoviruses species. Based on phenotypic symptoms observed in infected field plants, the names Sida yellow mosaic Yucatan virus (SiYMYuV) and Corchorus yellow vein Yucatan virus (CoYVYuV) were proposed. The SiYMYuV DNA-A shared the highest nucleotide identity (86{\%}) with the Okra yellow mosaic Mexico virus (OkYMMV). The complete DNA-B component shared the highest nucleotide identity (80{\%}) with CoYVYuV. The CoYVYuV DNA-A shared the highest nucleotide identity (84{\%}) with SiYMYuV. The 166-nt common region (CR) sequence for the DNA-A and DNA-B components of SiYMYuV shared a high nucleotide identity of 99{\%}, and the 151 nt of CoYVYuV CR shared 95{\%} of nucleotide identity. The organization and the iterated sequence of the putative AC1 binding site (located within the common region) of both isolates, were similar to that of the begomoviruses of the Western Hemisphere. Phylogenetic analyses placed the DNA-A and DNA-B of SiYMYuV and CoYVYuV in the clade containing the Abutilon mosaic virus (AbMV).",
keywords = "Begomovirus, Geminiviridae, Malvaceae, Molecular characterization, ssDNA virus, Weed",
author = "Cecilia Hern{\'a}ndez-Zepeda and Idris, {Ali M.} and Germ{\'a}n Carnevali and Brown, {Judith K} and Moreno-Valenzuela, {Oscar A.}",
year = "2007",
month = "10",
doi = "10.1007/s11262-007-0080-5",
language = "English (US)",
volume = "35",
pages = "369--377",
journal = "Virus Genes",
issn = "0920-8569",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in Yucatan, Mexico

AU - Hernández-Zepeda, Cecilia

AU - Idris, Ali M.

AU - Carnevali, Germán

AU - Brown, Judith K

AU - Moreno-Valenzuela, Oscar A.

PY - 2007/10

Y1 - 2007/10

N2 - Sida acuta and Corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the Yucatan Peninsula, Mexico. Total DNA was isolated from both plant species and used for the amplification, cloning, and sequencing of the Begomovirus genome. Nucleotide comparison of the complete DNA-A component isolated from S. acuta and C. siliquosus confirmed the presence of two distinct begomoviruses species. Based on phenotypic symptoms observed in infected field plants, the names Sida yellow mosaic Yucatan virus (SiYMYuV) and Corchorus yellow vein Yucatan virus (CoYVYuV) were proposed. The SiYMYuV DNA-A shared the highest nucleotide identity (86%) with the Okra yellow mosaic Mexico virus (OkYMMV). The complete DNA-B component shared the highest nucleotide identity (80%) with CoYVYuV. The CoYVYuV DNA-A shared the highest nucleotide identity (84%) with SiYMYuV. The 166-nt common region (CR) sequence for the DNA-A and DNA-B components of SiYMYuV shared a high nucleotide identity of 99%, and the 151 nt of CoYVYuV CR shared 95% of nucleotide identity. The organization and the iterated sequence of the putative AC1 binding site (located within the common region) of both isolates, were similar to that of the begomoviruses of the Western Hemisphere. Phylogenetic analyses placed the DNA-A and DNA-B of SiYMYuV and CoYVYuV in the clade containing the Abutilon mosaic virus (AbMV).

AB - Sida acuta and Corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the Yucatan Peninsula, Mexico. Total DNA was isolated from both plant species and used for the amplification, cloning, and sequencing of the Begomovirus genome. Nucleotide comparison of the complete DNA-A component isolated from S. acuta and C. siliquosus confirmed the presence of two distinct begomoviruses species. Based on phenotypic symptoms observed in infected field plants, the names Sida yellow mosaic Yucatan virus (SiYMYuV) and Corchorus yellow vein Yucatan virus (CoYVYuV) were proposed. The SiYMYuV DNA-A shared the highest nucleotide identity (86%) with the Okra yellow mosaic Mexico virus (OkYMMV). The complete DNA-B component shared the highest nucleotide identity (80%) with CoYVYuV. The CoYVYuV DNA-A shared the highest nucleotide identity (84%) with SiYMYuV. The 166-nt common region (CR) sequence for the DNA-A and DNA-B components of SiYMYuV shared a high nucleotide identity of 99%, and the 151 nt of CoYVYuV CR shared 95% of nucleotide identity. The organization and the iterated sequence of the putative AC1 binding site (located within the common region) of both isolates, were similar to that of the begomoviruses of the Western Hemisphere. Phylogenetic analyses placed the DNA-A and DNA-B of SiYMYuV and CoYVYuV in the clade containing the Abutilon mosaic virus (AbMV).

KW - Begomovirus

KW - Geminiviridae

KW - Malvaceae

KW - Molecular characterization

KW - ssDNA virus

KW - Weed

UR - http://www.scopus.com/inward/record.url?scp=34447634528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447634528&partnerID=8YFLogxK

U2 - 10.1007/s11262-007-0080-5

DO - 10.1007/s11262-007-0080-5

M3 - Article

C2 - 17638064

AN - SCOPUS:34447634528

VL - 35

SP - 369

EP - 377

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

IS - 2

ER -