Molecular characterization of insulin-like peptide genes and their expression in the African malaria mosquito, Anopheles gambiae

M. J.B. Krieger, N. Jahan, M. A. Riehle, C. Cao, M. R. Brown

Research output: Contribution to journalArticle

63 Scopus citations


Of the seven genes encoding insulin-like peptides (ILPs) in the mosquito, Anopheles gambiae, four are arrayed proximally as duplicate pairs on chromosome three. Amino acid substitutions encoded in the duplicate genes occur in the C peptide and not the B and A peptides. Except for one duplicated gene, sequence-specific transcripts for all other AgamILPs were obtained from female mosquitoes. Transcript expression of each AgamILP was determined by RT-PCR in the head, thorax, and abdomen of all life stages and both sexes of this mosquito. Two AgamILPs were ubiquitously expressed, suggesting a growth factor function, whereas the other AgamILPs were expressed primarily in heads, as confirmed by the immunostaining of ILPs in the neurosecretory cells of female brains, thus indicating a hormonal function.

Original languageEnglish (US)
Pages (from-to)305-315
Number of pages11
JournalInsect Molecular Biology
Issue number3
StatePublished - Jun 1 2004
Externally publishedYes



  • Bombyxin
  • Gene duplication
  • Peptide hormone

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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