Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors

Stephanie A. Freeman, Maria Reye Sierra Alvarez, Mahmut Altinbas, Jeremy Hollingsworth, Alfons J M Stams, Hauke Smidt

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel electrophoresis, cloning, and phylogenetic analysis. Bacterial and archaeal communities were examined over 450 days of operation treating ethanol (thermophilic reactor) or ethanol and later a simulated semiconductor manufacturing wastewater containing citrate, isopropanol, and polyethylene glycol 300 (mesophilic reactor), with and without the addition of copper(II). Analysis, of PCR-amplified 16S rRNA gene fragments using denaturing gradient gel electrophoresis revealed a defined shift in microbial diversity in both reactors following a change in substrate composition (mesophilic reactor) and in temperature of operation from 30°C to 55°C (thermophilic reactor). The addition of copper(II) to the influent of both reactors did not noticeably affect the composition of the bacterial or archaeal communities, which is in agreement with the very low soluble copper concentrations (3-310 μg l-1) present in the reactor contents as a consequence of extensive precipitation of copper with biogenic sulfides. Furthermore, clone library analysis confirmed the phylogenetic diversity of sulfate-reducing consortia in mesophilic and thermophilic sulfidogenic reactors operated with simple substrates.

Original languageEnglish (US)
Pages (from-to)161-177
Number of pages17
JournalBiodegradation
Volume19
Issue number2
DOIs
StatePublished - Apr 2008

Fingerprint

Sewage
Sulfates
Copper
microbial community
sludge
sulfate
Denaturing Gradient Gel Electrophoresis
Electrophoresis
Ethanol
Gels
Semiconductors
copper
2-Propanol
Cloning
Sulfides
Substrates
Waste Water
Chemical analysis
rRNA Genes
Citric Acid

Keywords

  • 16S rRNA gene clone library
  • Anaerobic wastewater treatment
  • Copper
  • DGGE
  • Ethanol
  • Methanogens
  • Sulfate reducing bacteria

ASJC Scopus subject areas

  • Biotechnology

Cite this

Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors. / Freeman, Stephanie A.; Sierra Alvarez, Maria Reye; Altinbas, Mahmut; Hollingsworth, Jeremy; Stams, Alfons J M; Smidt, Hauke.

In: Biodegradation, Vol. 19, No. 2, 04.2008, p. 161-177.

Research output: Contribution to journalArticle

Freeman, Stephanie A. ; Sierra Alvarez, Maria Reye ; Altinbas, Mahmut ; Hollingsworth, Jeremy ; Stams, Alfons J M ; Smidt, Hauke. / Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors. In: Biodegradation. 2008 ; Vol. 19, No. 2. pp. 161-177.
@article{20e74ea1837142d6834352f97bb91758,
title = "Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors",
abstract = "The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel electrophoresis, cloning, and phylogenetic analysis. Bacterial and archaeal communities were examined over 450 days of operation treating ethanol (thermophilic reactor) or ethanol and later a simulated semiconductor manufacturing wastewater containing citrate, isopropanol, and polyethylene glycol 300 (mesophilic reactor), with and without the addition of copper(II). Analysis, of PCR-amplified 16S rRNA gene fragments using denaturing gradient gel electrophoresis revealed a defined shift in microbial diversity in both reactors following a change in substrate composition (mesophilic reactor) and in temperature of operation from 30°C to 55°C (thermophilic reactor). The addition of copper(II) to the influent of both reactors did not noticeably affect the composition of the bacterial or archaeal communities, which is in agreement with the very low soluble copper concentrations (3-310 μg l-1) present in the reactor contents as a consequence of extensive precipitation of copper with biogenic sulfides. Furthermore, clone library analysis confirmed the phylogenetic diversity of sulfate-reducing consortia in mesophilic and thermophilic sulfidogenic reactors operated with simple substrates.",
keywords = "16S rRNA gene clone library, Anaerobic wastewater treatment, Copper, DGGE, Ethanol, Methanogens, Sulfate reducing bacteria",
author = "Freeman, {Stephanie A.} and {Sierra Alvarez}, {Maria Reye} and Mahmut Altinbas and Jeremy Hollingsworth and Stams, {Alfons J M} and Hauke Smidt",
year = "2008",
month = "4",
doi = "10.1007/s10532-007-9123-9",
language = "English (US)",
volume = "19",
pages = "161--177",
journal = "Biodegradation",
issn = "0923-9820",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors

AU - Freeman, Stephanie A.

AU - Sierra Alvarez, Maria Reye

AU - Altinbas, Mahmut

AU - Hollingsworth, Jeremy

AU - Stams, Alfons J M

AU - Smidt, Hauke

PY - 2008/4

Y1 - 2008/4

N2 - The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel electrophoresis, cloning, and phylogenetic analysis. Bacterial and archaeal communities were examined over 450 days of operation treating ethanol (thermophilic reactor) or ethanol and later a simulated semiconductor manufacturing wastewater containing citrate, isopropanol, and polyethylene glycol 300 (mesophilic reactor), with and without the addition of copper(II). Analysis, of PCR-amplified 16S rRNA gene fragments using denaturing gradient gel electrophoresis revealed a defined shift in microbial diversity in both reactors following a change in substrate composition (mesophilic reactor) and in temperature of operation from 30°C to 55°C (thermophilic reactor). The addition of copper(II) to the influent of both reactors did not noticeably affect the composition of the bacterial or archaeal communities, which is in agreement with the very low soluble copper concentrations (3-310 μg l-1) present in the reactor contents as a consequence of extensive precipitation of copper with biogenic sulfides. Furthermore, clone library analysis confirmed the phylogenetic diversity of sulfate-reducing consortia in mesophilic and thermophilic sulfidogenic reactors operated with simple substrates.

AB - The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel electrophoresis, cloning, and phylogenetic analysis. Bacterial and archaeal communities were examined over 450 days of operation treating ethanol (thermophilic reactor) or ethanol and later a simulated semiconductor manufacturing wastewater containing citrate, isopropanol, and polyethylene glycol 300 (mesophilic reactor), with and without the addition of copper(II). Analysis, of PCR-amplified 16S rRNA gene fragments using denaturing gradient gel electrophoresis revealed a defined shift in microbial diversity in both reactors following a change in substrate composition (mesophilic reactor) and in temperature of operation from 30°C to 55°C (thermophilic reactor). The addition of copper(II) to the influent of both reactors did not noticeably affect the composition of the bacterial or archaeal communities, which is in agreement with the very low soluble copper concentrations (3-310 μg l-1) present in the reactor contents as a consequence of extensive precipitation of copper with biogenic sulfides. Furthermore, clone library analysis confirmed the phylogenetic diversity of sulfate-reducing consortia in mesophilic and thermophilic sulfidogenic reactors operated with simple substrates.

KW - 16S rRNA gene clone library

KW - Anaerobic wastewater treatment

KW - Copper

KW - DGGE

KW - Ethanol

KW - Methanogens

KW - Sulfate reducing bacteria

UR - http://www.scopus.com/inward/record.url?scp=39749187249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39749187249&partnerID=8YFLogxK

U2 - 10.1007/s10532-007-9123-9

DO - 10.1007/s10532-007-9123-9

M3 - Article

C2 - 17479349

AN - SCOPUS:39749187249

VL - 19

SP - 161

EP - 177

JO - Biodegradation

JF - Biodegradation

SN - 0923-9820

IS - 2

ER -