Abstract
Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 531-538 |
| Number of pages | 8 |
| Journal | Journal of Virology |
| Volume | 60 |
| Issue number | 2 |
| State | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection. / Martinez, Jesse D; Jeor, S. C S.
In: Journal of Virology, Vol. 60, No. 2, 1986, p. 531-538.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection
AU - Martinez, Jesse D
AU - Jeor, S. C S
PY - 1986
Y1 - 1986
N2 - Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.
AB - Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022972462&partnerID=8YFLogxK
M3 - Article
VL - 60
SP - 531
EP - 538
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 2
ER -