Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection

Jesse D Martinez, S. C S Jeor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.

Original languageEnglish (US)
Pages (from-to)531-538
Number of pages8
JournalJournal of Virology
Volume60
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Human herpesvirus 5
Molecular Cloning
Cytomegalovirus
Post Transcriptional RNA Processing
molecular cloning
Complementary DNA
Clone Cells
RNA
clones
antibodies
Antibodies
beta-Galactosidase
beta-galactosidase
Infection
Gene Library
virion
cDNA libraries
infection
Virion
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection. / Martinez, Jesse D; Jeor, S. C S.

In: Journal of Virology, Vol. 60, No. 2, 1986, p. 531-538.

Research output: Contribution to journalArticle

@article{aad80ceb0fee4f85a7ea9541b8073b89,
title = "Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection",
abstract = "Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.",
author = "Martinez, {Jesse D} and Jeor, {S. C S}",
year = "1986",
language = "English (US)",
volume = "60",
pages = "531--538",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Molecular cloning and analysis of three cDNA clones homologous to human cytomegalovirus RNAs present during late infection

AU - Martinez, Jesse D

AU - Jeor, S. C S

PY - 1986

Y1 - 1986

N2 - Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.

AB - Three virus-specific clones were isolated from a cDNA library synthesized from human cytomegalovirus (AD169)-infected cell RNA and cloned into the expression vector lambda gt11. These clones, designated C3, D10, and H10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. By using the cloned cDNA, we were able to identify the transcripts that code for each gene product and study the kinetics of expression during permissive infection. Our results suggest that at least two of the RNAs undergo posttranscriptional processing and appear in infected cells at immediate-early times. The authentic C3 gene product was identified by probing Western blots with antibody prepared against fusion protein fpC3.

UR - http://www.scopus.com/inward/record.url?scp=0022972462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022972462&partnerID=8YFLogxK

M3 - Article

VL - 60

SP - 531

EP - 538

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

ER -