Molecular cloning and expression analysis of soluble and membrane-bound trehalase genes in the cotton bollworm, Helicoverpa armigera

Long Ma, Wu Dai, Xianchun Li, Yalin Zhang, Chunni Zhang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Trehalase plays a significant role in various physiological processes in insects. In this study, we cloned and characterized a soluble trehalase gene (HaTreh-1) and a membrane-bound trehalase gene (HaTreh-2) from Helicoverpa armigera, a serious polyphagous pest of crops. HaTreh-1 contained an open reading frame of 1716. bp that encodes a protein of 572 amino acids. HaTreh-2 has an open reading frame of 1938. bp, encoding a protein of 646 amino acids. Sequence alignment and phylogenetic analysis of the two putative proteins revealed that HaTreh-1 and HaTreh-2 belong to soluble and membrane-bound trehalase groups, respectively. Quantitative real-time PCR (qPCR) analyses of the spatiotemporal expression pattern of HaTreh in H. armigera revealed that HaTreh-1 was expressed mainly in the midgut, with lower expression in the integument and head, Malpighian tubules, trachea, and fat body. The expression levels of HaTreh-2 were detected in all 6 tissues, and HaTreh-2 was mainly expressed in the midgut and head. Expression of HaTreh-1 was higher throughout the larval stages, but lower on days 1 and 2 of the pupal stage. Expression of HaTreh-2 was higher during the 4th- and 5th-instar larval stages. Taken together, these results suggest that HaTreh-1 and HaTreh-2 have different functions in various developmental stages and tissues.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalJournal of Asia-Pacific Entomology
Volume18
Issue number2
DOIs
StatePublished - Jun 1 2015

Keywords

  • Cloning
  • Expression pattern
  • Helicoverpa armigera
  • Membrane-bound trehalase
  • Quantitative real-time PCR
  • Soluble trehalase

ASJC Scopus subject areas

  • Insect Science

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