Molecular cloning and expression of mRNAs encoding H1 histone and an H1 histone-like sequences in root tips of pea (Pisum sativum L.)

Ho Hyung Woo, Lindy A. Brigham, Martha C Hawes

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Two cDNA clones representing mRNAs, highly expressed in pea root tips, were isolated by mRNA differential display. Ribonuclease protection analyses showed different patterns of expression of these two messages in several pea tissues. Sequence analysis showed that the first clone, PsH1b-40, has 100% homology with a previously isolated H1 histone cDNA, PsH1b. However, it has an additional 30 nt at the 3′ end which is absent in PsH1b, suggesting possible multiple polyadenylation sites in the same mRNA. The second clone, PsH1b-41, encodes a deduced 19.5 kDa protein of 185 amino acids with an isoelectric point of 11.5. The putative globular domain of the encoded protein showed 67-71% residue identity with globular domains of 28 kDa pea PsH1b H1 histone and Arabidopsis thaliana H1-1 H1 histone. It has 9 repeating motifs of (T/S)XXK. In the C-terminal domain, there are four lysine-rich repeating motifs of SXK(T/S)PXKKXK which may be involved in chromatin condensation and decondensation. Southern blot analysis of nuclear DNA shows that PsH1-41 belongs to a multigene family.

Original languageEnglish (US)
Pages (from-to)1143-1147
Number of pages5
JournalPlant Molecular Biology
Volume28
Issue number6
DOIs
StatePublished - Sep 1995

Fingerprint

Meristem
Cloning
Peas
Molecular Cloning
root tips
histones
Histones
Pisum sativum
molecular cloning
peas
Clone Cells
clones
Messenger RNA
Complementary DNA
Polyadenylation
ribonucleases
Isoelectric Point
Gene Expression Profiling
isoelectric point
Ribonucleases

Keywords

  • globular domain
  • H1 histone
  • pea
  • repeating motif of amino acids

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Molecular cloning and expression of mRNAs encoding H1 histone and an H1 histone-like sequences in root tips of pea (Pisum sativum L.). / Woo, Ho Hyung; Brigham, Lindy A.; Hawes, Martha C.

In: Plant Molecular Biology, Vol. 28, No. 6, 09.1995, p. 1143-1147.

Research output: Contribution to journalArticle

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