Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli

T. K. Karjalainen, D. G. Evans, Magdalene "Maggie" So, C. H. Lee

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The colonization factor antigen I (CFA/I) gene has been isolated and sequenced. The amino acid sequence of CFA/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA/I protein since they are not present in the protein sequence. Among the remaining amino acids, only 2 are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA/I gene promoter. No sequence homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of 8 A's are located 45 bp downstream from the termination codon of the CFA/I gene; this region may be a p-independent transcriptional terminator.

Original languageEnglish (US)
Pages (from-to)1126-1130
Number of pages5
JournalInfection and Immunity
Volume57
Issue number4
StatePublished - 1989
Externally publishedYes

Fingerprint

Molecular Cloning
Escherichia coli
Base Pairing
Genes
Initiator Codon
Amino Acids
Amino Acid Sequence
Inverted Repeat Sequences
Proteins
Terminator Codon
Asparagine
Sequence Homology
Protein Sorting Signals
Aspartic Acid
Alanine
Serine
colonization factor antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli. / Karjalainen, T. K.; Evans, D. G.; So, Magdalene "Maggie"; Lee, C. H.

In: Infection and Immunity, Vol. 57, No. 4, 1989, p. 1126-1130.

Research output: Contribution to journalArticle

@article{c7881aa33bcf41b2b0a2f4bd54f291b9,
title = "Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli",
abstract = "The colonization factor antigen I (CFA/I) gene has been isolated and sequenced. The amino acid sequence of CFA/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA/I protein since they are not present in the protein sequence. Among the remaining amino acids, only 2 are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA/I gene promoter. No sequence homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of 8 A's are located 45 bp downstream from the termination codon of the CFA/I gene; this region may be a p-independent transcriptional terminator.",
author = "Karjalainen, {T. K.} and Evans, {D. G.} and So, {Magdalene {"}Maggie{"}} and Lee, {C. H.}",
year = "1989",
language = "English (US)",
volume = "57",
pages = "1126--1130",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli

AU - Karjalainen, T. K.

AU - Evans, D. G.

AU - So, Magdalene "Maggie"

AU - Lee, C. H.

PY - 1989

Y1 - 1989

N2 - The colonization factor antigen I (CFA/I) gene has been isolated and sequenced. The amino acid sequence of CFA/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA/I protein since they are not present in the protein sequence. Among the remaining amino acids, only 2 are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA/I gene promoter. No sequence homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of 8 A's are located 45 bp downstream from the termination codon of the CFA/I gene; this region may be a p-independent transcriptional terminator.

AB - The colonization factor antigen I (CFA/I) gene has been isolated and sequenced. The amino acid sequence of CFA/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA/I protein since they are not present in the protein sequence. Among the remaining amino acids, only 2 are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA/I gene promoter. No sequence homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of 8 A's are located 45 bp downstream from the termination codon of the CFA/I gene; this region may be a p-independent transcriptional terminator.

UR - http://www.scopus.com/inward/record.url?scp=0024584283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024584283&partnerID=8YFLogxK

M3 - Article

VL - 57

SP - 1126

EP - 1130

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 4

ER -