Molecular cloning, functional characterization, tissue distribution, and chromosomal localization of a human, small intestinal sodium-phosphate (Na+- P(i)) transporter (SLC34A2)

Hua Xu, Liqun Bai, James F. Collins, Fayez K. Ghishan

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

Phosphate plays a crucial role in cellular metabolism, and its homeostatic regulation in intestinal and renal epithelia is critical. Apically expressed sodium-phosphate (Na+-P(i)) transporters play a critical role in this regulation. We have isolated a cDNA (HGMW-approved symbol SLC34A2) encoding a novel human small intestinal Na+-P(i) transporter. The cDNA is shown to be 4135 bp in length with an open reading frame that predicts a 689-amino-acid polypeptide. The putative protein has 76% homology to mouse intestinal type II Na+-P(i) transporter (Na/Pi-IIb) and lower homologics with renal type II Na+-P(i) transporters. Northern blots showed a singular transcript of 5.0 kb in human lung, small intestine, and kidney. Computer analysis suggests a protein with 11 transmembrane domains and several potential posttranslational modification sites. Functional characterization in Xenopus laevis oocytes showed that this cDNA encodes a functional Na+-P(i) transporter. Furthermore, the gene encoding this cDNA was mapped to human chromosome 4p15.1-p15.3 by the FISH method.

Original languageEnglish (US)
Pages (from-to)281-284
Number of pages4
JournalGenomics
Volume62
Issue number2
DOIs
StatePublished - Dec 1 1999

ASJC Scopus subject areas

  • Genetics

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