Molecular cloning of a cotton phosphatase gene and its functional characterization

Shi You Lu, Guang Rong Zhao, Ai Min Wu, M. A. Jenks, Shuping Zhang, Jin Yuan Liu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In this study, we report isolation of a phosphatase gene designated GhHL1 from cotton and its functional characterization. GhHL1 transcripts were detected in all cotton tissues examined. Southern blotting analysis indicated that it exists in multiple-copies. Biochemical analysis showed that GhHL1 was Mg 2+-dependent and cation-sensitive. Purified recombinant GhHL1 protein dephosphorylated both 3′,5′-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3′,5′-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities. Overexpression of GhHL1 complemented yeast hal2 mutant and enhanced yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response. Taken together, these results show that GhHL1 is a functional and good candidate gene, which might be used to improve salt tolerance in plants.

Original languageEnglish (US)
Pages (from-to)85-94
Number of pages10
JournalBiochemistry (Moscow)
Volume75
Issue number1
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

Keywords

  • Cotton
  • Enzyme activity
  • Functional characterization
  • Phosphatase
  • Salt tolerance

ASJC Scopus subject areas

  • Biochemistry

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