Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

L. Bai, J. F. Collins, H. Xu, L. Xu, F. K. Ghishan

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.

Original languageEnglish (US)
Pages (from-to)42-45
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1522
Issue number1
DOIs
StatePublished - Nov 11 2001

Keywords

  • Caco-2 cell
  • Gene regulation
  • Intestine
  • OKP cell
  • Ram-1
  • Sodium-phosphate cotransporter

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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