Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Kayo Arima, James F. Collins, Eric R. Hines, Liqun Bai, Fayez K. Ghishan

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1494
Issue number1-2
DOIs
StatePublished - Nov 15 2000

Keywords

  • Intestine
  • Promoter analysis
  • Sodium-phosphate transporter

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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