Molecular cloning of rabbit organic cation transporter rbOCT2 and functional comparisons with rbOCT1

Xiaohong Zhang, Kristen K. Evans, Stephen H. Wright

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Multiple organic cation transporters (OCTs) are present in rabbit kidney and may play different functional roles. We cloned rabbit OCT2 (rbOCT2) and compared its function with that of rabbit OCT1 (rbOCT1). In transiently transfected COS-7 cells, rbOCT1 and rbOCT2 mediated uptake of [3H]tetraethylammonium (TEA) with Kt values of 188 and 125 μM, respectively, n-Tetraalkylammonium compounds showed similar affinities for the two homologs, with IC50 values for inhibition of OCT1- and OCT2-mediated [3H]TEA transport, respectively, of 4,538 and 1,395 μM for tetramethylammonium, 88.5 and 3.9 μM for tetrapropylammonium, 13.9 and 5.3 μM for tetrabutylammonium, and 8.8 and 7.6 μM for tetrapentylammonium. However, the transporters had very different affinities for cimetidine (CIM): IC50 of 916 and 5.7 μM for rbOCT1 and rbOCT2, respectively. CIM inhibition of TEA uptake into single S2 segments of rabbit proximal tubule was used to estimate the contributions of OCT1 and OCT2 to basolateral organic cation uptake. The median IC50 for CIM inhibition of TEA uptake was 12.3 μM, suggesting that OCT2 is the major contributor to basolateral organic cation transport in the S2 segment of proximal tubule in rabbit kidney.

Original languageEnglish (US)
Pages (from-to)F124-F133
JournalAmerican Journal of Physiology - Renal Physiology
Volume283
Issue number1 52-1
StatePublished - Jul 4 2002

    Fingerprint

Keywords

  • Kidney
  • Proximal tubule
  • Rabbit organic cation transporter
  • Tetraethylammonium
  • Uptake

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this