Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+exchanger (SLC9A2)

Fayez K. Ghishan, Susan M. Knobel, Marshall Summar

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The Na+/H+exchangers belong to a family of transport proteins involved in intracellular pH regulation and vectorial sodium transport across various epithelial tissues. We have cloned a unique isoform (NHE-2) by screening a rat intestinal cDNA library utilizing a human NHE-1 fragment. In this report we describe molecular cloning of the human Na+/H+exchanger (NHE-2, HGMW-approved symbol SLC9A2). The cDNA encodes a protein of 698 amino acid residues. Human NHE-2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands. Hydropathy plot indicates that the translated protein is predicted to have 10 transmembrane domains with three potential N-glycosylation sites and four potential cAMP- and cGMP-dependent protein kinase phosphorylation sites. This cDNA maps the location of the gene encoding NHE-2 to human chromosome 2q11.2.

Original languageEnglish (US)
Pages (from-to)25-30
Number of pages6
JournalGenomics
Volume30
Issue number1
DOIs
StatePublished - Nov 1995
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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