Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2)

James F. Collins, Tatsuya Honda, Susan Knobel, Nada M. Bulus, Jon Conary, Ray DuBois, Fayez K Ghishan

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The present studies demonstrate cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger which was isolated from a rat intestinal cDNA library. The cloned cDNA recognizes two transcripts in poly(A)+ RNA from the stomach, jejunum, ileum, liver, large intestine, and uterus. Based on deduced amino acid sequences, this clone shares sequence homology with the other known Na+/H+ exchanger isoforms (NHE-1, NHE-3, and NHE-4) except for its 5′ end. Overall, the protein exhibits 47.8%, 41.2%, and 56.2% amino acid sequence identity to NHE-1, NHE-3, and NHE-4, respectively. The hydropathy profile of the predicted protein shows 10 transmembrane domains, suggesting a protein with transport characteristics. The tissue distribution differs from that of the other Na+/H+ exchanger isoforms. The cDNA hybridizes to two closely related transcripts in the mRNA of these tissues, which suggests that the predominant transcript of this clone is alternatively spliced. Transfection of this cDNA into Na+/H+ exchanger-deficient mutant fibroblasts (PS120 cells) results in functional Na+/H+ exchange activity. These data suggest that we have cloned a member of the Na+/H+ exchanger family with tissue-specific expression. We suggest the designation of NHE-2 for this Na+/H+ exchanger.

Original languageEnglish (US)
Pages (from-to)3938-3942
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number9
StatePublished - May 1 1993
Externally publishedYes

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Sodium-Hydrogen Antiporter
Molecular Cloning
Tissue Distribution
Complementary DNA
Amino Acid Sequence
Protein Isoforms
Clone Cells
Messenger RNA
Large Intestine
Protein Transport
Jejunum
Sequence Homology
Gene Library
Ileum
Uterus
Transfection
Organism Cloning
Stomach
Proteins
Fibroblasts

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2). / Collins, James F.; Honda, Tatsuya; Knobel, Susan; Bulus, Nada M.; Conary, Jon; DuBois, Ray; Ghishan, Fayez K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 9, 01.05.1993, p. 3938-3942.

Research output: Contribution to journalArticle

Collins, James F. ; Honda, Tatsuya ; Knobel, Susan ; Bulus, Nada M. ; Conary, Jon ; DuBois, Ray ; Ghishan, Fayez K. / Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2). In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 9. pp. 3938-3942.
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