Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes

Reza Riahi, Kathleen E. MacH, Ruchika Mohan, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Rapid, specific, and sensitive detection of bacterial pathogens is essential toward clinical management of infectious diseases. Traditional approaches for pathogen detection, however, often require time-intensive bacterial culture and amplification procedures. Herein, a microparticle enhanced double-stranded DNA probe is demonstrated for rapid species-specific detection of bacterial 16S rRNA. In this molecular assay, the binding of the target sequence to the fluorophore conjugated probe thermodynamically displaces the quencher probe and allows the fluorophore to fluoresce. By incorporation of streptavidin-coated microparticles to localize the biotinylated probes, the sensitivity of the assay can be improved by 3 orders of magnitude. The limit of detection of the assay is as few as eight bacteria without target amplification and is highly specific against other common pathogens. Its applicability toward clinical diagnostics is demonstrated by directly identifying bacterial pathogens in urine samples from patients with urinary tract infections.

Original languageEnglish (US)
Pages (from-to)6349-6354
Number of pages6
JournalAnalytical Chemistry
Volume83
Issue number16
DOIs
StatePublished - Aug 15 2011

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DNA Probes
Pathogens
Assays
Fluorophores
DNA
Amplification
Streptavidin
Bacteria

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes. / Riahi, Reza; MacH, Kathleen E.; Mohan, Ruchika; Liao, Joseph C.; Wong, Pak Kin.

In: Analytical Chemistry, Vol. 83, No. 16, 15.08.2011, p. 6349-6354.

Research output: Contribution to journalArticle

Riahi, Reza ; MacH, Kathleen E. ; Mohan, Ruchika ; Liao, Joseph C. ; Wong, Pak Kin. / Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes. In: Analytical Chemistry. 2011 ; Vol. 83, No. 16. pp. 6349-6354.
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