Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest

R. Husband, E. A. Herre, S. L. Turner, Rachel Elizabeth Gallery, J. P W Young

Research output: Contribution to journalArticle

291 Citations (Scopus)

Abstract

We have used molecular techniques to investigate the diversity and distribution of the arbuscular mycorrhizal (AM) fungi colonizing tree seedling roots in the tropical forest on Barro Colorado Island (BCI), Republic of Panama. In the first year, we sampled newly emergent seedlings of the understory treelet Faramea occidentalis and the canopy emergent Tetragastris panamensis, from mixed seedling carpets at each of two sites. The following year we sampled surviving seedlings from these cohorts. The roots of 48 plants were analysed using AM fungal-specific primers to amplify and clone partial small subunit (SSU) ribosomal RNA gene sequences. Over 1300 clones were screened for random fragment length polymorphism (RFLP) variation and 7% of these were sequenced. Compared with AM fungal communities sampled from temperate habitats using the same method, the overall diversity was high, with a total of 30 AM fungal types identified. Seventeen of these types have not been recorded previously, with the remainder being similar to types reported from temperate habitats. The tropical mycorrhizal population showed significant spatial heterogeneity and nonrandom associations with the different hosts. Moreover there was a strong shift in the mycorrhizal communities over time. AM fungal types that were dominant in the newly germinated seedlings were almost entirely replaced by previously rare types in the surviving seedlings the following year. The high diversity and huge variation detected across time points, sites and hosts, implies that the AM fungal types are ecologically distinct and thus may have the potential to influence recruitment and host composition in tropical forests.

Original languageEnglish (US)
Pages (from-to)2669-2678
Number of pages10
JournalMolecular Ecology
Volume11
Issue number12
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Ribosomal RNA
Fungi
Polymorphism
Seedlings
space and time
tropical forests
tropical forest
mycorrhizal fungi
Genes
seedling
fungus
seedlings
Chemical analysis
Ecosystem
clone
Clone Cells
clones
Panama
Plant Roots
fungal communities

Keywords

  • AM1
  • Arbuscular mycorrhiza
  • Community composition
  • Diversity
  • SSU
  • Tropical forest

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest. / Husband, R.; Herre, E. A.; Turner, S. L.; Gallery, Rachel Elizabeth; Young, J. P W.

In: Molecular Ecology, Vol. 11, No. 12, 01.12.2002, p. 2669-2678.

Research output: Contribution to journalArticle

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