Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress

A. G. Blaszczak, R. Smith, A. Gutierrez, D. W. Galbraith, J. Janda, C. Vanier, E. M. Wozniak

Research output: ResearchConference contribution

  • 1 Citations

Abstract

Abiotic stress, including drought, cold, high temperature and UV radiation, produce common responses in plant tissue, resulting in the overproduction of Reactive Oxygen Species (ROS). ROS molecules damage cell membranes and disrupt plant metabolism. Our previous experiments indicated that naturally chelated/complexed foliar nutritional supplement products reduce the amount of ROS molecules formed under stress conditions. In this study, we investigated the short-term effect of the novel proprietary biostimulant CYT31 on gene expression in plants exposed to drought using Arabidopsis thaliana grown under controlled conditions. Plants were sprayed with biostimulant CYT31 (treated) or water (control). One hour after spraying, plants were exposed to drought conditions for one, two or four hours. Tissue was collected for two-color microarray and cell damage analysis. Tissue damage was evaluated using an electrolyte leakage-detection method. Drought stress up-regulated over 245 genes involved in water deprivation, redox and stress response. The gene response was influenced by the severity of the stress and correlated with tissue damage. The up-regulation of these genes was significantly altered in the tissue treated with biostimulant CYT31, suggesting the activation of several mechanisms leading to the reduction of tissue injury. Gene activation was confirmed by quantitative polymerase chain reaction (qPCR) analysis. This paper describes a proposed model for metabolic pathways activated by biostimulant CYT31 in plants exposed to drought stress.

LanguageEnglish (US)
Title of host publication2nd World Congress on the Use of Biostimulants in Agriculture
PublisherInternational Society for Horticultural Science
Pages85-92
Number of pages8
Volume1148
ISBN (Electronic)9789462611382
DOIs
StatePublished - Nov 18 2016

Publication series

NameActa Horticulturae
Volume1148
ISSN (Print)0567-7572

Fingerprint

mechanism of action
water stress
tissues
reactive oxygen species
drought
genes
water deprivation
gene activation
spraying
abiotic stress
electrolytes
ultraviolet radiation
cell membranes
dietary supplements
plant tissues
biochemical pathways
stress response
quantitative polymerase chain reaction
Arabidopsis thaliana
gene expression

Keywords

  • Arabidopsis thaliana
  • Cytozyme
  • Gene expression
  • Microarray
  • QPCR
  • Reactive oxygen species (ROS)
  • Water deprivation pathways
  • Western blotting

ASJC Scopus subject areas

  • Horticulture

Cite this

Blaszczak, A. G., Smith, R., Gutierrez, A., Galbraith, D. W., Janda, J., Vanier, C., & Wozniak, E. M. (2016). Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. In 2nd World Congress on the Use of Biostimulants in Agriculture (Vol. 1148, pp. 85-92). (Acta Horticulturae; Vol. 1148). International Society for Horticultural Science. DOI: 10.17660/ActaHortic.2016.1148.10

Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. / Blaszczak, A. G.; Smith, R.; Gutierrez, A.; Galbraith, D. W.; Janda, J.; Vanier, C.; Wozniak, E. M.

2nd World Congress on the Use of Biostimulants in Agriculture. Vol. 1148 International Society for Horticultural Science, 2016. p. 85-92 (Acta Horticulturae; Vol. 1148).

Research output: ResearchConference contribution

Blaszczak, AG, Smith, R, Gutierrez, A, Galbraith, DW, Janda, J, Vanier, C & Wozniak, EM 2016, Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. in 2nd World Congress on the Use of Biostimulants in Agriculture. vol. 1148, Acta Horticulturae, vol. 1148, International Society for Horticultural Science, pp. 85-92. DOI: 10.17660/ActaHortic.2016.1148.10
Blaszczak AG, Smith R, Gutierrez A, Galbraith DW, Janda J, Vanier C et al. Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. In 2nd World Congress on the Use of Biostimulants in Agriculture. Vol. 1148. International Society for Horticultural Science. 2016. p. 85-92. (Acta Horticulturae). Available from, DOI: 10.17660/ActaHortic.2016.1148.10
Blaszczak, A. G. ; Smith, R. ; Gutierrez, A. ; Galbraith, D. W. ; Janda, J. ; Vanier, C. ; Wozniak, E. M./ Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. 2nd World Congress on the Use of Biostimulants in Agriculture. Vol. 1148 International Society for Horticultural Science, 2016. pp. 85-92 (Acta Horticulturae).
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