Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences

Barry M Pryor, R. L. Gilbertson

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

To elucidate relationships among Alternaria, Ulocladium, and Stemphylium species, nuclear internal transcribed spacer (ITS) and mitochondrial small subunit (SSU) ribosomal DNA (rDNA) sequences from 18 Alternaria, four Ulocladium and four Stemphylium spp. were determined and compared. Phylogenetic analysis of the ITS and SSU rDNA sequences, performed by the neighbour joining and maximum parsimony methods, revealed that the Stemphylium spp. were phylogenetically distinct from the Alternaria and Ulocladium spp. Most Alternaria spp. and the Ulocladium spp. were placed together in a large Alternaria/Ulocladium clade. Within this large clade, the Alternaria spp. clustered into several distinct species-clades, most of which correlated with species-groups previously established based upon morphological characteristics. The Ulocladium spp. were placed into two species-clades, each of which also included Alternaria spp. A. longissima was distantly related to the other Alternaria spp., as well as the Ulocladium and Stemphylium spp. Based upon ITS and nuclear 18S rDNA sequence identities, A. longissima was most closely related to Leptosphaeria.

Original languageEnglish (US)
Pages (from-to)1312-1321
Number of pages10
JournalMycological Research
Volume104
Issue number11
DOIs
StatePublished - 2000
Externally publishedYes

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Alternaria
Ulocladium
Ribosomal DNA
ribosomal DNA
internal transcribed spacers
Fungi
fungus
Stemphylium
phylogenetics
nucleotide sequences
DNA
fungi
phylogeny
Leptosphaeria
analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. / Pryor, Barry M; Gilbertson, R. L.

In: Mycological Research, Vol. 104, No. 11, 2000, p. 1312-1321.

Research output: Contribution to journalArticle

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