Molecular requirements for assembly and function of a minimized human integrin α(IIb)β3

Brian S Mckay, Douglas S. Annis, Shigenori Honda, Douglas Christie, Thomas J. Kunicki

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Integrin subunit compatibility within and between species plays a major role in heterodimer assembly and ligand specificity. As an example, human α(IIb) pairs only with human β3 and does not assemble a heterodimer with β3 from other species. We use interspecies subunit chimeras to identify molecular requirements for subunit compatibility and show that species- restricted heterodimer assembly depends on a unique hexapeptide VGSDNH in an extended loop of the hypothetical human β3 MIDAS domain. This allows us to express α(IIb)(1-233) and β3(111-318) as a soluble, mini-integrin that retains RGD-dependent ligand recognition. Thus, in the case of one integrin, α(IIb)β3, the molecular requirements for integrin subunit compatibility and ligand recognition are intimately related.

Original languageEnglish (US)
Pages (from-to)30544-30547
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number48
DOIs
StatePublished - 1996
Externally publishedYes

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Integrins
Ligands

ASJC Scopus subject areas

  • Biochemistry

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Molecular requirements for assembly and function of a minimized human integrin α(IIb)β3. / Mckay, Brian S; Annis, Douglas S.; Honda, Shigenori; Christie, Douglas; Kunicki, Thomas J.

In: Journal of Biological Chemistry, Vol. 271, No. 48, 1996, p. 30544-30547.

Research output: Contribution to journalArticle

Mckay, Brian S ; Annis, Douglas S. ; Honda, Shigenori ; Christie, Douglas ; Kunicki, Thomas J. / Molecular requirements for assembly and function of a minimized human integrin α(IIb)β3. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 48. pp. 30544-30547.
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