Molecular requirements for assembly and function of a minimized human integrin α(IIb)β3

Brian S. McKay, Douglas S. Annis, Shigenori Honda, Douglas Christie, Thomas J. Kunicki

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Integrin subunit compatibility within and between species plays a major role in heterodimer assembly and ligand specificity. As an example, human α(IIb) pairs only with human β3 and does not assemble a heterodimer with β3 from other species. We use interspecies subunit chimeras to identify molecular requirements for subunit compatibility and show that species- restricted heterodimer assembly depends on a unique hexapeptide VGSDNH in an extended loop of the hypothetical human β3 MIDAS domain. This allows us to express α(IIb)(1-233) and β3(111-318) as a soluble, mini-integrin that retains RGD-dependent ligand recognition. Thus, in the case of one integrin, α(IIb)β3, the molecular requirements for integrin subunit compatibility and ligand recognition are intimately related.

Original languageEnglish (US)
Pages (from-to)30544-30547
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number48
DOIs
StatePublished - Dec 14 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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