Molecular tools for the study of titin's differential expression

T. Centner, F. Fougerousse, A. Freiburg, C. Witt, J. S. Beckmann, Hendrikus "Henk" Granzier, K. Trombitás, Carol Gregorio, S. Labeit, Greaser, Bullard, Linke, Pollack, Trinick

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Although vertebrate genomes appear to contain only one titin gene, a large variety of quite distinct titin isoforms are expressed in striated muscle tissues. The isoforms appear to be generated by a series of complex, not yet fully characterized differential splicing mechanisms. Here, we provide an overview of the titin-specific antibodies that have been raised by our laboratory to study individual differentially expressed isoforms of titin. The staining patterns obtained in different tissues will contribute to the identification of both the particular titin isoforms that are expressed in the different tissues, as well as their intracellular distributions. In addition, antibodies to titin that are available are rapidly allowing for the refinement of our knowledge of titin's elastic spring properties. Knowledge of the nature and structure of vertebrate titins that may also be expressed in nonmuscle tissues may be broadened using these antibodies.

Original languageEnglish (US)
Pages (from-to)35-52
Number of pages18
JournalAdvances in Experimental Medicine and Biology
Volume481
StatePublished - 2000
Externally publishedYes

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Connectin
Protein Isoforms
Tissue
Vertebrates
Antibodies
Genes
Striated Muscle
Muscle
Genome
Staining and Labeling
Muscles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Centner, T., Fougerousse, F., Freiburg, A., Witt, C., Beckmann, J. S., Granzier, H. H., ... Trinick (2000). Molecular tools for the study of titin's differential expression. Advances in Experimental Medicine and Biology, 481, 35-52.

Molecular tools for the study of titin's differential expression. / Centner, T.; Fougerousse, F.; Freiburg, A.; Witt, C.; Beckmann, J. S.; Granzier, Hendrikus "Henk"; Trombitás, K.; Gregorio, Carol; Labeit, S.; Greaser; Bullard; Linke; Pollack; Trinick.

In: Advances in Experimental Medicine and Biology, Vol. 481, 2000, p. 35-52.

Research output: Contribution to journalArticle

Centner, T, Fougerousse, F, Freiburg, A, Witt, C, Beckmann, JS, Granzier, HH, Trombitás, K, Gregorio, C, Labeit, S, Greaser, Bullard, Linke, Pollack & Trinick 2000, 'Molecular tools for the study of titin's differential expression', Advances in Experimental Medicine and Biology, vol. 481, pp. 35-52.
Centner T, Fougerousse F, Freiburg A, Witt C, Beckmann JS, Granzier HH et al. Molecular tools for the study of titin's differential expression. Advances in Experimental Medicine and Biology. 2000;481:35-52.
Centner, T. ; Fougerousse, F. ; Freiburg, A. ; Witt, C. ; Beckmann, J. S. ; Granzier, Hendrikus "Henk" ; Trombitás, K. ; Gregorio, Carol ; Labeit, S. ; Greaser ; Bullard ; Linke ; Pollack ; Trinick. / Molecular tools for the study of titin's differential expression. In: Advances in Experimental Medicine and Biology. 2000 ; Vol. 481. pp. 35-52.
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