Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley

Z. Neslihan Ozturk, Valentina Talamé, Michael Deyholos, Christine B. Michalowski, David W. Galbraith, Nermin Gozukirmizi, Roberto Tuberosa, Hans J. Bohnert

Research output: Contribution to journalArticlepeer-review

435 Scopus citations

Abstract

Responses to drought and salinity in barley (Hordeum vulgare L. cv. Tokak) were monitored by microarray hybridization of 1463 DNA elements derived from cDNA libraries of 6 and 10 h drought-stressed plants. Functional identities indicated that many cDNAs in these libraries were associated with drought stress. About 38% of the transcripts were novel and functionally unknown. Hybridization experiments were analyzed for drought- and salinity-regulated sequences, with significant changes defined as a deviation from the control exceeding 2.5-fold. Responses of transcripts showed stress-dependent expression patterns and time courses. Nearly 15% of all transcripts were either up- or down-regulated under drought stress, while NaCl led to a change in 5% of the transcripts (24 h, 150 mM NaCl). Transcripts that showed significant up-regulation under drought stress are exemplified by jasmonate-responsive, metallothionein-like, late-embryogenesis-abundant (LEA) and ABA-responsive proteins. Most drastic down-regulation in a category was observed for photosynthesis-related functions. Up-regulation under both drought and salt stress was restricted to ESTs for metallothionein-like and LEA proteins, while increases in ubiquitin-related transcripts characterized salt stress. A number of functionally unknown transcripts from cDNA libraries of drought-stressed plants showed up-regulation by drought but down-regulation by salt stress, documenting how precisely transcript profiles report different growth conditions and environments.

Original languageEnglish (US)
Pages (from-to)551-573
Number of pages23
JournalPlant Molecular Biology
Volume48
Issue number5-6
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Drought stress
  • Hordeum vulgate
  • Microarray hybridization
  • Salinity stress
  • Stress-regulated transcripts

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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