Monocyte recruitment to endothelial cells in response to oscillatory shear stress

Tzung K. Hsiai, Sung K. Cho, Pak K. Wong, Mike Ing, Adler Salazar, Alex Sevanian, Mohamad Navab, Linda L. Demer, Chih Ming Ho

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Leukocyte recruitment to endothelial cells is a critical event in inflammatory responses. The spatial, temporal gradients of shear stress, topology, and outcome of cellular interactions that underlie these responses have so far been inferred from static imaging of tissue sections or studies of statically cultured cells. In this report, we developed micro-electromechanical systems (MEMS) sensors, comparable to a single endothelial cell (EC) in size, to link real-time shear stress with monocyte/EC binding kinetics in a complex flow environment, simulating the moving and unsteady separation point at the arterial bifurcation with high spatial and temporal resolution. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm2 at a time-averaged shear stress (τave) = 0 and 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Our study quantified individual monocyte/EC binding kinetics in terms of displacement and velocity profiles. Oscillatory flow induces up-regulation of adhesion molecules and cytokines to mediate monocyte/EC interactions over a dynamic range of shear stress ± 2.6 dyn/cm2 (P = 0.50, n= 10).

Original languageEnglish (US)
Pages (from-to)1648-1657
Number of pages10
JournalFASEB Journal
Volume17
Issue number12
DOIs
StatePublished - Sep 2003

Keywords

  • Cell tracking velocimetry
  • Endothelial cells
  • Micro-electromechanical systems (MEMS)
  • Monocytes
  • Shear stress sensors

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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  • Cite this

    Hsiai, T. K., Cho, S. K., Wong, P. K., Ing, M., Salazar, A., Sevanian, A., Navab, M., Demer, L. L., & Ho, C. M. (2003). Monocyte recruitment to endothelial cells in response to oscillatory shear stress. FASEB Journal, 17(12), 1648-1657. https://doi.org/10.1096/fj.02-1064com