Monoselenophosphate: Synthesis, characterization, and identity with the prokaryotic biological selenium donor, compound SePX

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Abstract

A labile, selenium donor compound required for synthesis of selenium-dependent enzymes and seleno-tRN As is formed from ATP and selenide by the SELD enzyme. This compound, tentatively identified as a selenophosphate [Veres, Z., Tsai, L., Scholz, T. D., Politino, M., Balaban, R. S., & Stadtman, T. C. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 2975-2979], is indistinguishable from chemically prepared monoselenophosphate by 31P NMR spectroscopy and ion pairing HPLC. Furthermore, addition of chemically prepared monoselenophosphate caused a dose-dependent decrease in the amount of 75Se incorporated into tRNAs from 75SePX generated in situ by SELD enzyme. A procedure is described for the chemical synthesis of monoselenophosphate in which the readily prepared (MeO)3PSe is converted in quantitative yield to (TMSO)3PSe followed by complete cleavage of the latter to monoselenophosphate in oxygen-free aqueous buffer. The chemical properties of chemically synthesized monoselenophosphate are described.

Original languageEnglish (US)
Pages (from-to)12555-12559
Number of pages5
JournalBiochemistry
StatePublished - 1993

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Selenium Compounds
Selenium
Enzymes
Transfer RNA
Chemical properties
Nuclear magnetic resonance spectroscopy
selenophosphate
Buffers
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
High Pressure Liquid Chromatography
Ions
Oxygen

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{3020accac24c4c098de6776a28020199,
title = "Monoselenophosphate: Synthesis, characterization, and identity with the prokaryotic biological selenium donor, compound SePX",
abstract = "A labile, selenium donor compound required for synthesis of selenium-dependent enzymes and seleno-tRN As is formed from ATP and selenide by the SELD enzyme. This compound, tentatively identified as a selenophosphate [Veres, Z., Tsai, L., Scholz, T. D., Politino, M., Balaban, R. S., & Stadtman, T. C. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 2975-2979], is indistinguishable from chemically prepared monoselenophosphate by 31P NMR spectroscopy and ion pairing HPLC. Furthermore, addition of chemically prepared monoselenophosphate caused a dose-dependent decrease in the amount of 75Se incorporated into tRNAs from 75SePX generated in situ by SELD enzyme. A procedure is described for the chemical synthesis of monoselenophosphate in which the readily prepared (MeO)3PSe is converted in quantitative yield to (TMSO)3PSe followed by complete cleavage of the latter to monoselenophosphate in oxygen-free aqueous buffer. The chemical properties of chemically synthesized monoselenophosphate are described.",
author = "Glass, {Richard S}",
year = "1993",
language = "English (US)",
pages = "12555--12559",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",

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TY - JOUR

T1 - Monoselenophosphate

T2 - Synthesis, characterization, and identity with the prokaryotic biological selenium donor, compound SePX

AU - Glass, Richard S

PY - 1993

Y1 - 1993

N2 - A labile, selenium donor compound required for synthesis of selenium-dependent enzymes and seleno-tRN As is formed from ATP and selenide by the SELD enzyme. This compound, tentatively identified as a selenophosphate [Veres, Z., Tsai, L., Scholz, T. D., Politino, M., Balaban, R. S., & Stadtman, T. C. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 2975-2979], is indistinguishable from chemically prepared monoselenophosphate by 31P NMR spectroscopy and ion pairing HPLC. Furthermore, addition of chemically prepared monoselenophosphate caused a dose-dependent decrease in the amount of 75Se incorporated into tRNAs from 75SePX generated in situ by SELD enzyme. A procedure is described for the chemical synthesis of monoselenophosphate in which the readily prepared (MeO)3PSe is converted in quantitative yield to (TMSO)3PSe followed by complete cleavage of the latter to monoselenophosphate in oxygen-free aqueous buffer. The chemical properties of chemically synthesized monoselenophosphate are described.

AB - A labile, selenium donor compound required for synthesis of selenium-dependent enzymes and seleno-tRN As is formed from ATP and selenide by the SELD enzyme. This compound, tentatively identified as a selenophosphate [Veres, Z., Tsai, L., Scholz, T. D., Politino, M., Balaban, R. S., & Stadtman, T. C. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 2975-2979], is indistinguishable from chemically prepared monoselenophosphate by 31P NMR spectroscopy and ion pairing HPLC. Furthermore, addition of chemically prepared monoselenophosphate caused a dose-dependent decrease in the amount of 75Se incorporated into tRNAs from 75SePX generated in situ by SELD enzyme. A procedure is described for the chemical synthesis of monoselenophosphate in which the readily prepared (MeO)3PSe is converted in quantitative yield to (TMSO)3PSe followed by complete cleavage of the latter to monoselenophosphate in oxygen-free aqueous buffer. The chemical properties of chemically synthesized monoselenophosphate are described.

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C2 - 8251472

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JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

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