Morphological changes with Na+-free fluid absorption in isolated perfused snake tubules

William H Dantzler, O. H. Brokl, Raymond B Nagle, D. J. Welling, L. W. Welling

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Snake (Thamnophis spp.) proximal renal tubules were perfused and bathed in vitro either with medium containing sodium or with medium in which the sodium was replaced with choline. Net fluid absorption was measured by changes in volume marker concentration, and cell volumes and cell membrane surface areas were measured by ultrastructural morphometric methods. Net fluid absorption did not differ significantly in the presence or absence of sodium. However, during the 20-25-min perfusion in the absence of sodium, significant morphological changes took place. The volume of the cells, doubled and the volume of the intercellular spaces nearly quintupled. The areas of the lateral and apical cell membranes approximately doubled, but their surface densities remained essentially constant. Therefore the larger cells in the absence of sodium had proportionally enlarged surface areas, so that the volume-to-surface area ratio remained constant. These morphological changes occurred concomitantly with the maintenance of net fluid absorption and might play a permissive role in such maintenance in the absence of sodium.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume251
Edition1
StatePublished - 1986

Fingerprint

Snakes
Sodium
Cell Size
Maintenance
Cell Membrane
Colubridae
Proximal Kidney Tubule
Extracellular Space
Choline
Perfusion

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Dantzler, W. H., Brokl, O. H., Nagle, R. B., Welling, D. J., & Welling, L. W. (1986). Morphological changes with Na+-free fluid absorption in isolated perfused snake tubules. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (1 ed., Vol. 251)

Morphological changes with Na+-free fluid absorption in isolated perfused snake tubules. / Dantzler, William H; Brokl, O. H.; Nagle, Raymond B; Welling, D. J.; Welling, L. W.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 251 1. ed. 1986.

Research output: Chapter in Book/Report/Conference proceedingChapter

Dantzler, WH, Brokl, OH, Nagle, RB, Welling, DJ & Welling, LW 1986, Morphological changes with Na+-free fluid absorption in isolated perfused snake tubules. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1 edn, vol. 251.
Dantzler WH, Brokl OH, Nagle RB, Welling DJ, Welling LW. Morphological changes with Na+-free fluid absorption in isolated perfused snake tubules. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1 ed. Vol. 251. 1986
Dantzler, William H ; Brokl, O. H. ; Nagle, Raymond B ; Welling, D. J. ; Welling, L. W. / Morphological changes with Na+-free fluid absorption in isolated perfused snake tubules. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 251 1. ed. 1986.
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