Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with alternaria late blight of pistachio

Barry M Pryor, Themis J. Michailides

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Alternaria isolates were obtained from various pistachio tissues collected in five orchards in California. For all isolates, morphological characteristics of the colony and sporulation apparatus were determined and compared with those of representative isolates of A. alternata, A. tenuissima, A. arborescens, and A. infectoria. A selection of the pistachio isolates and the representative Alternaria isolates were evaluated for pathogenicity to pistachio. Molecular characteristics of these isolates were determined using random amplified polymorphism DNA (RAPD) analysis, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis of nuclear intergenic spacer rDNA, and sequence analysis of nuclear internal transcribed spacer (ITS) rDNA. Based on morphological characteristics, the pistachio isolates were grouped as identical or very similar to either A. alternata, A. tenuissima, A. arborescens, or A. infectoria. Isolates from the alternata, tenuissima, and arborescens species-groups were pathogenic to pistachio and no significant differences in pathogenicity were observed. Isolates from the infectoria species-group were only weakly pathogenic to pistachio. Based on cluster analysis of RAPD and PCR-RFLP data, three distinct clusters were evident; the infectoria cluster, the arborescens cluster, and a combined alternata/tenuissima cluster. Based on analysis of ITS sequence data, the infectoria species-group was phylogenetically distinct from the other species-groups. Isolates of the alternata, tenuissima, and arborescens species-groups comprised a monophyletic clade in which the three species-groups could not be further resolved.

Original languageEnglish (US)
Pages (from-to)406-416
Number of pages11
JournalPhytopathology
Volume92
Issue number4
StatePublished - 2002

Fingerprint

pistachios
Alternaria
Phytophthora infestans
intergenic DNA
internal transcribed spacers
restriction fragment length polymorphism
pathogenicity
polymerase chain reaction
genetic polymorphism
DNA
sporulation
polymorphism
cluster analysis
orchards
sequence analysis

Keywords

  • DNA fingerprinting
  • Pistacia vera
  • Taxonomy

ASJC Scopus subject areas

  • Plant Science

Cite this

Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with alternaria late blight of pistachio. / Pryor, Barry M; Michailides, Themis J.

In: Phytopathology, Vol. 92, No. 4, 2002, p. 406-416.

Research output: Contribution to journalArticle

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