Morphology and ultrastructure of the bacterial receptacle in Steinernema nematodes (Nematoda

Steinernematidae)

Sam Kyu Kim, Yolanda Flores-Lara, S Patricia Stock

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Infective juveniles of entomopathogenic nematodes in the genus . Steinernema harbor symbiotic bacteria, . Xenorhabdus spp., in a discrete structure located in the anterior portion of the intestine known as the 'bacterial receptacle' (formerly known as the bacterial or intestinal vesicle). The receptacle itself is a structured environment in which the bacteria are spatially restricted. Inside this receptacle, bacterial symbionts are protected from the environment and grow to fill the receptacle. Until now, no comparative study across different . Steinernema spp. has been undertaken to investigate if morphological variation in this structure exists at the interspecific level. In this study, we examined the bacterial receptacles of 25 . Steinernema spp. representatives of the currently accepted five evolutionary clades. Our observations confirmed the bacterial receptacle is a modification of the two most anterior cells of the ventricular portion of the intestine. Size of the bacterial receptacle varied across the examined species. . Steinernema monticolum (clade II) had the largest receptacle of all examined species (average: 46. ×. 17. μm) and . S. rarum (no clade affiliation) was noted as the species with the smallest observed receptacle (average: 8. ×. 5. μm). At the morphological level, species can be grouped into two categories based on the presence or absence of vesicle within the receptacle. The receptacles of all examined species harbored an intravesicular structure (IVS) with variable morphology. All examined taxa members of the 'feltiae' (clade III) and 'intermedium' (clade II) clades were characterized by having a vesicle. This structure was also observed in . S. diaprepesi (clade V), . S. riobrave (clade IV) and . S. monticolum (clade I).

Original languageEnglish (US)
Pages (from-to)366-374
Number of pages9
JournalJournal of Invertebrate Pathology
Volume110
Issue number3
DOIs
StatePublished - Jul 2012

Fingerprint

Steinernematidae
Steinernema
ultrastructure
nematode
Nematoda
vesicle
intestines
Xenorhabdus
nematode larvae
entomopathogenic nematodes
bacteria
bacterium
symbionts
symbiont
comparative study
harbor
cells

Keywords

  • Bacterial receptacle
  • DIC microscopy
  • Interspecific variation
  • Morphology
  • Steinernema
  • TEM
  • Ultrastructure
  • Xenorhabdus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Morphology and ultrastructure of the bacterial receptacle in Steinernema nematodes (Nematoda : Steinernematidae). / Kim, Sam Kyu; Flores-Lara, Yolanda; Stock, S Patricia.

In: Journal of Invertebrate Pathology, Vol. 110, No. 3, 07.2012, p. 366-374.

Research output: Contribution to journalArticle

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abstract = "Infective juveniles of entomopathogenic nematodes in the genus . Steinernema harbor symbiotic bacteria, . Xenorhabdus spp., in a discrete structure located in the anterior portion of the intestine known as the 'bacterial receptacle' (formerly known as the bacterial or intestinal vesicle). The receptacle itself is a structured environment in which the bacteria are spatially restricted. Inside this receptacle, bacterial symbionts are protected from the environment and grow to fill the receptacle. Until now, no comparative study across different . Steinernema spp. has been undertaken to investigate if morphological variation in this structure exists at the interspecific level. In this study, we examined the bacterial receptacles of 25 . Steinernema spp. representatives of the currently accepted five evolutionary clades. Our observations confirmed the bacterial receptacle is a modification of the two most anterior cells of the ventricular portion of the intestine. Size of the bacterial receptacle varied across the examined species. . Steinernema monticolum (clade II) had the largest receptacle of all examined species (average: 46. ×. 17. μm) and . S. rarum (no clade affiliation) was noted as the species with the smallest observed receptacle (average: 8. ×. 5. μm). At the morphological level, species can be grouped into two categories based on the presence or absence of vesicle within the receptacle. The receptacles of all examined species harbored an intravesicular structure (IVS) with variable morphology. All examined taxa members of the 'feltiae' (clade III) and 'intermedium' (clade II) clades were characterized by having a vesicle. This structure was also observed in . S. diaprepesi (clade V), . S. riobrave (clade IV) and . S. monticolum (clade I).",
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