Morphology of microtubules grown in agarose gels: Effect of diffusion and confinement

Javier S. Castro, Pierre A Deymier, Shane D. Smith, James B. Hoying

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Morphology of microtubules (MT) grown in crowded environment in the form of high viscosity fluids containing agarose were presented. Agarose influences the diffusion coefficient of molecules in high viscosity solutions and gels. The morphology of MTs were evolved from numerous short, straight MTs to longer MTs to fewer long bundles of MTs that were curved or contorted. Field emission scanning electron microscope (FESEM) showed that the porosity of gel was characterized by large pores separated by a denser network of agarose filaments. The large pores exhibited diameters in the order of hundreds of nanometers, whereas the denser network had small pores with diameters that were not greater than a few tens of nanometers. Experimental investigation demonstrated that the morphology of MTs grown in gels was directly dependent on the tubulin diffusion coefficient.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalAdvanced Materials
Volume20
Issue number1
DOIs
StatePublished - Jan 7 2008

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Sepharose
Gels
Viscosity
Tubulin
Field emission
Electron microscopes
Porosity
Scanning
Molecules
Fluids

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Morphology of microtubules grown in agarose gels : Effect of diffusion and confinement. / Castro, Javier S.; Deymier, Pierre A; Smith, Shane D.; Hoying, James B.

In: Advanced Materials, Vol. 20, No. 1, 07.01.2008, p. 183-188.

Research output: Contribution to journalArticle

Castro, Javier S. ; Deymier, Pierre A ; Smith, Shane D. ; Hoying, James B. / Morphology of microtubules grown in agarose gels : Effect of diffusion and confinement. In: Advanced Materials. 2008 ; Vol. 20, No. 1. pp. 183-188.
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