Morphometric modeling of olfactory circuits in the insect antennal lobe

I. Simulations of spiking local interneurons

Thomas A. Christensen, Giampaolo D'Alessandro, Joceline C Lega, John G Hildebrand

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Inhibitory local interneurons (LNs) play a critical role in shaping the output of olfactory glomeruli in both the olfactory bulb of vertebrates and the antennal lobe of insects and other invertebrates. In order to examine how the complex geometry of LNs may affect signaling in the antennal lobe, we constructed detailed multi-compartmental models of single LNs from the sphinx moth, Manduca sexta, using morphometric data from confocal-microscopic images. Simulations clearly revealed a directionality in LNs that impeded the propagation of injected currents from the sub-micron-diameter glomerular dendrites toward the much larger-diameter integrating segment (IS) in the coarse neuropil. Furthermore, the addition of randomly-firing synapses distributed across the LN dendrites (simulating the noisy baseline activity of afferent input recorded from LNs in the odor-free state) led to a significant depolarization of the LN. Thus the background activity typically recorded from LNs in vivo could influence synaptic integration and spike transformation in LNs through voltage-dependent mechanisms. Other model manipulations showed that active currents inserted into the IS can help synchronize the activation of inhibitory synapses in glomeruli across the antennal lobe. These data, therefore, support experimental findings suggesting that spiking inhibitory LNs can operate as multifunctional units under different ambient odor conditions. At low odor intensities, (i.e. subthreshold for IS spiking), they participate in local, mostly intra-glomerular processing. When activated by elevated odor concentrations, however, the same neurons will fire overshooting action potentials, resulting in the spread of inhibition more globally across the antennal lobe. Modulation of the passive and active properties of LNs may, therefore, be a deciding factor in defining the multi-glomerular representations of odors in the brain.

Original languageEnglish (US)
Pages (from-to)143-153
Number of pages11
JournalBioSystems
Volume61
Issue number2-3
DOIs
StatePublished - Jul 8 2001

Fingerprint

antennal lobe
interneurons
Odors
Interneurons
odor
Insects
insect
insects
Networks (circuits)
Modeling
modeling
simulation
Simulation
odors
Depolarization
Olfactory Bulb
dendrites
Dendrite
Synapse
synapse

Keywords

  • Glomeruli
  • Modulation
  • Odor processing
  • Olfaction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology
  • Drug Discovery

Cite this

Morphometric modeling of olfactory circuits in the insect antennal lobe : I. Simulations of spiking local interneurons. / Christensen, Thomas A.; D'Alessandro, Giampaolo; Lega, Joceline C; Hildebrand, John G.

In: BioSystems, Vol. 61, No. 2-3, 08.07.2001, p. 143-153.

Research output: Contribution to journalArticle

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