Abstract
Toxoplasma gondii infection occurs through the oral route, but we lack important information about how the parasite interacts with the host immune system in the intestine.We used two-photon laserscanning microscopy in conjunction with a mouse model of oral T. gondii infection to address this issue. T. gondii established discrete foci of infection in the small intestine, eliciting the recruitment and transepithelial migration of neutrophils and inflammatory monocytes. Neutrophils accounted for a high proportion of actively invaded cells, and we provide evidence for a role for transmigrating neutrophils and other immune cells in the spread of T. gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine.
| Original language | English (US) |
|---|---|
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 110 |
| Issue number | 21 |
| DOIs | |
| State | Published - May 21 2013 |
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Keywords
- Dynamic imaging
- Gut
- Mucosal immunology
- Neutrophil motility
ASJC Scopus subject areas
- General
Cite this
Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread. / Coombes, Janine L.; Charsar, Brittany A.; Han, Seong Ji; Halkias, Joanna; Chan, Shiao Wei; Koshy, Anita A; Striepen, Boris; Robey, Ellen A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 21, 21.05.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread
AU - Coombes, Janine L.
AU - Charsar, Brittany A.
AU - Han, Seong Ji
AU - Halkias, Joanna
AU - Chan, Shiao Wei
AU - Koshy, Anita A
AU - Striepen, Boris
AU - Robey, Ellen A.
PY - 2013/5/21
Y1 - 2013/5/21
N2 - Toxoplasma gondii infection occurs through the oral route, but we lack important information about how the parasite interacts with the host immune system in the intestine.We used two-photon laserscanning microscopy in conjunction with a mouse model of oral T. gondii infection to address this issue. T. gondii established discrete foci of infection in the small intestine, eliciting the recruitment and transepithelial migration of neutrophils and inflammatory monocytes. Neutrophils accounted for a high proportion of actively invaded cells, and we provide evidence for a role for transmigrating neutrophils and other immune cells in the spread of T. gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine.
AB - Toxoplasma gondii infection occurs through the oral route, but we lack important information about how the parasite interacts with the host immune system in the intestine.We used two-photon laserscanning microscopy in conjunction with a mouse model of oral T. gondii infection to address this issue. T. gondii established discrete foci of infection in the small intestine, eliciting the recruitment and transepithelial migration of neutrophils and inflammatory monocytes. Neutrophils accounted for a high proportion of actively invaded cells, and we provide evidence for a role for transmigrating neutrophils and other immune cells in the spread of T. gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine.
KW - Dynamic imaging
KW - Gut
KW - Mucosal immunology
KW - Neutrophil motility
UR - http://www.scopus.com/inward/record.url?scp=84878116280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878116280&partnerID=8YFLogxK
U2 - 10.1073/pnas.1220272110
DO - 10.1073/pnas.1220272110
M3 - Article
C2 - 23650399
AN - SCOPUS:84878116280
VL - 110
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 21
ER -