Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread

Janine L. Coombes, Brittany A. Charsar, Seong Ji Han, Joanna Halkias, Shiao Wei Chan, Anita A Koshy, Boris Striepen, Ellen A. Robey

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Toxoplasma gondii infection occurs through the oral route, but we lack important information about how the parasite interacts with the host immune system in the intestine.We used two-photon laserscanning microscopy in conjunction with a mouse model of oral T. gondii infection to address this issue. T. gondii established discrete foci of infection in the small intestine, eliciting the recruitment and transepithelial migration of neutrophils and inflammatory monocytes. Neutrophils accounted for a high proportion of actively invaded cells, and we provide evidence for a role for transmigrating neutrophils and other immune cells in the spread of T. gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number21
DOIs
StatePublished - May 21 2013

Fingerprint

Toxoplasmosis
Toxoplasma
Small Intestine
Parasites
Neutrophils
Intestines
Transendothelial and Transepithelial Migration
Photons
Monocytes
Microscopy
Immune System
Infection

Keywords

  • Dynamic imaging
  • Gut
  • Mucosal immunology
  • Neutrophil motility

ASJC Scopus subject areas

  • General

Cite this

Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread. / Coombes, Janine L.; Charsar, Brittany A.; Han, Seong Ji; Halkias, Joanna; Chan, Shiao Wei; Koshy, Anita A; Striepen, Boris; Robey, Ellen A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 21, 21.05.2013.

Research output: Contribution to journalArticle

Coombes, Janine L. ; Charsar, Brittany A. ; Han, Seong Ji ; Halkias, Joanna ; Chan, Shiao Wei ; Koshy, Anita A ; Striepen, Boris ; Robey, Ellen A. / Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 21.
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AU - Striepen, Boris

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