Mouse hepatitis virus is cleared from the central nervous systems of mice lacking perforin-mediated cytolysis

Mark T Lin, Stephen A. Stohlman, David R. Hinton

Research output: Contribution to journalArticle

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Abstract

Perforin-deficient [perforin (-/-)] mice were infected with two strains of JHM virus (JHMV) to analyze the role of perforin-mediated cytotoxicity in acute lethal and subacute central nervous system (CNS) infections. During both acute and subacute infections, the overall mortality of the perforin (- /-) mice was not different from that of the controls. Perforin (-/-) mice survived longer than the controls, consistent with reduced morbidity. Both strains of virus were cleared from the perforin (-/-) mice as in the controls; however, the rate of clearance was delayed in the perforin (-/-) mice, indicating that perforin-mediated cytolysis is involved in viral clearance. The absence of perforin-mediated cytolysis did not prevent encephalomyelitis or extensive demyelination. Cells undergoing apoptosis were detected in the CNS of both the perforin (-/-) and control groups, indicating that perforin is not essential for programmed cell death. Neutralizing antibodies were not detected in either group of mice until day 9 postinfection, when the majority of the virus had been cleared. These data further confirm the importance of cell-mediated cytotoxicity and suggest that additional components of the immune response contribute to the clearance of JHMV from the CNS.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalJournal of Virology
Volume71
Issue number1
StatePublished - 1997
Externally publishedYes

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Murine hepatitis virus
cytolysis
Neurologic Mutant Mice
Perforin
central nervous system
Central Nervous System
mice
Viruses
viruses
cytotoxicity
apoptosis
Encephalomyelitis
Central Nervous System Infections
Demyelinating Diseases
encephalitis
Neutralizing Antibodies
neutralizing antibodies
infection
morbidity
mouse perforin

ASJC Scopus subject areas

  • Immunology

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Mouse hepatitis virus is cleared from the central nervous systems of mice lacking perforin-mediated cytolysis. / Lin, Mark T; Stohlman, Stephen A.; Hinton, David R.

In: Journal of Virology, Vol. 71, No. 1, 1997, p. 383-391.

Research output: Contribution to journalArticle

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abstract = "Perforin-deficient [perforin (-/-)] mice were infected with two strains of JHM virus (JHMV) to analyze the role of perforin-mediated cytotoxicity in acute lethal and subacute central nervous system (CNS) infections. During both acute and subacute infections, the overall mortality of the perforin (- /-) mice was not different from that of the controls. Perforin (-/-) mice survived longer than the controls, consistent with reduced morbidity. Both strains of virus were cleared from the perforin (-/-) mice as in the controls; however, the rate of clearance was delayed in the perforin (-/-) mice, indicating that perforin-mediated cytolysis is involved in viral clearance. The absence of perforin-mediated cytolysis did not prevent encephalomyelitis or extensive demyelination. Cells undergoing apoptosis were detected in the CNS of both the perforin (-/-) and control groups, indicating that perforin is not essential for programmed cell death. Neutralizing antibodies were not detected in either group of mice until day 9 postinfection, when the majority of the virus had been cleared. These data further confirm the importance of cell-mediated cytotoxicity and suggest that additional components of the immune response contribute to the clearance of JHMV from the CNS.",
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