Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome

Norman Arnheim, Peter Seperack, Julian Banerji, Rhonda B. Lang, Roger Miesfeld, Kenneth B. Marcu

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

When a cloned 6 kb Eco RI-Sal I fragment of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse μ, α and γ2b immunoglobulin CH genes. One region was localized 3′ to the μ coding sequence, an area which has been identified as an intervening sequence between the secreted Cμ heavy chain terminus and the C terminal portion of the membrane-bound Cμ heavy chain.

Original languageEnglish (US)
Pages (from-to)179-185
Number of pages7
JournalCell
Volume22
Issue number1
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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