Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin C(H) genes and elsewhere throughout the genome

N. Arnheim, P. Seperack, J. Banerji, R. B. Lang, Roger Miesfeld, K. B. Marcu

Research output: Chapter in Book/Report/Conference proceedingChapter

42 Citations (Scopus)

Abstract

When a cloned 6 kb Eco RI-Sal I fragment of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse μ, α and γ2b immunoglobulin C(H) genes. One region was localized 3' to the μ coding sequence, an area which has been identified as an intervening sequence between the secreted C(μ) heavy chain terminus and the C terminal portion of the membrane-bound C(μ) heavy chain.

Original languageEnglish (US)
Title of host publicationCell
Pages179-185
Number of pages7
Volume22
Edition1 I
DOIs
StatePublished - 1980
Externally publishedYes

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Ribosomal DNA
Immunoglobulins
Genome
Sequence Homology
Genes
Deoxyribonuclease EcoRI
Intergenic DNA
Gene Library
Bacteriophages
Introns
In Situ Hybridization
Clone Cells
Membranes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin C(H) genes and elsewhere throughout the genome. / Arnheim, N.; Seperack, P.; Banerji, J.; Lang, R. B.; Miesfeld, Roger; Marcu, K. B.

Cell. Vol. 22 1 I. ed. 1980. p. 179-185.

Research output: Chapter in Book/Report/Conference proceedingChapter

Arnheim, N. ; Seperack, P. ; Banerji, J. ; Lang, R. B. ; Miesfeld, Roger ; Marcu, K. B. / Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin C(H) genes and elsewhere throughout the genome. Cell. Vol. 22 1 I. ed. 1980. pp. 179-185
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AU - Miesfeld, Roger

AU - Marcu, K. B.

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