Mouthwash as a low-cost and safe specimen transport medium for human papillomavirus DNA testing of cervicovaginal specimens

Philip E. Castle, Mark Sadorra, Francisco A Garcia, Allison P. Cullen, Attila T. Lorincz, Amy L Mitchell, Denise Whitby, Ronald Chuke, Janet R. Kornegay

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The usefulness of mouthwash as a transport medium for cervical specimens for carcinogenic human papillomavirus (HPV) DNA testing has not been evaluated. Two cervical specimens were collected from each of 34 patients, with one placed in mouthwash (Scope, Proctor and Gamble, Inc.) and the other in a liquid cytology medium commonly used for HPV DNA testing in alternating order. Paired specimens were tested by a PCR assay for carcinogenic HPV and a PCR HPV genotyping assay for 37 HPV types at 0, 3, and 6 weeks after collection; the results of the HPV genotyping assay were categorized into HPV risk groups according to cancer risk (HPV-16 > HPV-18 > other carcinogenic HPV types > noncarcinogenic HPV types > negative). After 4 months of storage, specimens were tested using a second, non-PCR test for carcinogenic HPV. We observed a ≥94% total agreement and κ values of ≥0.88 between media at each time point for PCR-detected carcinogenic HPV. We observed a ≥74% total agreement, ≥0.62 unweighted κ, and ≥0.75 linearly weighted κ between media at each time point for PCR-detected HPV cancer risk category. Finally, we observed an 88% total agreement and κ of 0.77 between media for carcinogenic HPV detection using a second test after 4 months of storage. We suggest that mouthwash might be used as a low-cost, safe, nonflammable storage and transport medium for cervical specimens for HPV DNA testing in cervical cancer screening programs.

Original languageEnglish (US)
Pages (from-to)840-843
Number of pages4
JournalCancer Epidemiology Biomarkers and Prevention
Volume16
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Mouthwashes
Costs and Cost Analysis
DNA
Polymerase Chain Reaction
Human papillomavirus 18
Human papillomavirus 16
Early Detection of Cancer

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Mouthwash as a low-cost and safe specimen transport medium for human papillomavirus DNA testing of cervicovaginal specimens. / Castle, Philip E.; Sadorra, Mark; Garcia, Francisco A; Cullen, Allison P.; Lorincz, Attila T.; Mitchell, Amy L; Whitby, Denise; Chuke, Ronald; Kornegay, Janet R.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 16, No. 4, 04.2007, p. 840-843.

Research output: Contribution to journalArticle

Castle, Philip E. ; Sadorra, Mark ; Garcia, Francisco A ; Cullen, Allison P. ; Lorincz, Attila T. ; Mitchell, Amy L ; Whitby, Denise ; Chuke, Ronald ; Kornegay, Janet R. / Mouthwash as a low-cost and safe specimen transport medium for human papillomavirus DNA testing of cervicovaginal specimens. In: Cancer Epidemiology Biomarkers and Prevention. 2007 ; Vol. 16, No. 4. pp. 840-843.
@article{0fb2186aa2ef453c869920a83c246e9f,
title = "Mouthwash as a low-cost and safe specimen transport medium for human papillomavirus DNA testing of cervicovaginal specimens",
abstract = "The usefulness of mouthwash as a transport medium for cervical specimens for carcinogenic human papillomavirus (HPV) DNA testing has not been evaluated. Two cervical specimens were collected from each of 34 patients, with one placed in mouthwash (Scope, Proctor and Gamble, Inc.) and the other in a liquid cytology medium commonly used for HPV DNA testing in alternating order. Paired specimens were tested by a PCR assay for carcinogenic HPV and a PCR HPV genotyping assay for 37 HPV types at 0, 3, and 6 weeks after collection; the results of the HPV genotyping assay were categorized into HPV risk groups according to cancer risk (HPV-16 > HPV-18 > other carcinogenic HPV types > noncarcinogenic HPV types > negative). After 4 months of storage, specimens were tested using a second, non-PCR test for carcinogenic HPV. We observed a ≥94{\%} total agreement and κ values of ≥0.88 between media at each time point for PCR-detected carcinogenic HPV. We observed a ≥74{\%} total agreement, ≥0.62 unweighted κ, and ≥0.75 linearly weighted κ between media at each time point for PCR-detected HPV cancer risk category. Finally, we observed an 88{\%} total agreement and κ of 0.77 between media for carcinogenic HPV detection using a second test after 4 months of storage. We suggest that mouthwash might be used as a low-cost, safe, nonflammable storage and transport medium for cervical specimens for HPV DNA testing in cervical cancer screening programs.",
author = "Castle, {Philip E.} and Mark Sadorra and Garcia, {Francisco A} and Cullen, {Allison P.} and Lorincz, {Attila T.} and Mitchell, {Amy L} and Denise Whitby and Ronald Chuke and Kornegay, {Janet R.}",
year = "2007",
month = "4",
doi = "10.1158/1055-9965.EPI-06-0909",
language = "English (US)",
volume = "16",
pages = "840--843",
journal = "Cancer Epidemiology Biomarkers and Prevention",
issn = "1055-9965",
publisher = "American Association for Cancer Research Inc.",
number = "4",

}

TY - JOUR

T1 - Mouthwash as a low-cost and safe specimen transport medium for human papillomavirus DNA testing of cervicovaginal specimens

AU - Castle, Philip E.

AU - Sadorra, Mark

AU - Garcia, Francisco A

AU - Cullen, Allison P.

AU - Lorincz, Attila T.

AU - Mitchell, Amy L

AU - Whitby, Denise

AU - Chuke, Ronald

AU - Kornegay, Janet R.

PY - 2007/4

Y1 - 2007/4

N2 - The usefulness of mouthwash as a transport medium for cervical specimens for carcinogenic human papillomavirus (HPV) DNA testing has not been evaluated. Two cervical specimens were collected from each of 34 patients, with one placed in mouthwash (Scope, Proctor and Gamble, Inc.) and the other in a liquid cytology medium commonly used for HPV DNA testing in alternating order. Paired specimens were tested by a PCR assay for carcinogenic HPV and a PCR HPV genotyping assay for 37 HPV types at 0, 3, and 6 weeks after collection; the results of the HPV genotyping assay were categorized into HPV risk groups according to cancer risk (HPV-16 > HPV-18 > other carcinogenic HPV types > noncarcinogenic HPV types > negative). After 4 months of storage, specimens were tested using a second, non-PCR test for carcinogenic HPV. We observed a ≥94% total agreement and κ values of ≥0.88 between media at each time point for PCR-detected carcinogenic HPV. We observed a ≥74% total agreement, ≥0.62 unweighted κ, and ≥0.75 linearly weighted κ between media at each time point for PCR-detected HPV cancer risk category. Finally, we observed an 88% total agreement and κ of 0.77 between media for carcinogenic HPV detection using a second test after 4 months of storage. We suggest that mouthwash might be used as a low-cost, safe, nonflammable storage and transport medium for cervical specimens for HPV DNA testing in cervical cancer screening programs.

AB - The usefulness of mouthwash as a transport medium for cervical specimens for carcinogenic human papillomavirus (HPV) DNA testing has not been evaluated. Two cervical specimens were collected from each of 34 patients, with one placed in mouthwash (Scope, Proctor and Gamble, Inc.) and the other in a liquid cytology medium commonly used for HPV DNA testing in alternating order. Paired specimens were tested by a PCR assay for carcinogenic HPV and a PCR HPV genotyping assay for 37 HPV types at 0, 3, and 6 weeks after collection; the results of the HPV genotyping assay were categorized into HPV risk groups according to cancer risk (HPV-16 > HPV-18 > other carcinogenic HPV types > noncarcinogenic HPV types > negative). After 4 months of storage, specimens were tested using a second, non-PCR test for carcinogenic HPV. We observed a ≥94% total agreement and κ values of ≥0.88 between media at each time point for PCR-detected carcinogenic HPV. We observed a ≥74% total agreement, ≥0.62 unweighted κ, and ≥0.75 linearly weighted κ between media at each time point for PCR-detected HPV cancer risk category. Finally, we observed an 88% total agreement and κ of 0.77 between media for carcinogenic HPV detection using a second test after 4 months of storage. We suggest that mouthwash might be used as a low-cost, safe, nonflammable storage and transport medium for cervical specimens for HPV DNA testing in cervical cancer screening programs.

UR - http://www.scopus.com/inward/record.url?scp=34247549723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247549723&partnerID=8YFLogxK

U2 - 10.1158/1055-9965.EPI-06-0909

DO - 10.1158/1055-9965.EPI-06-0909

M3 - Article

C2 - 17416781

AN - SCOPUS:34247549723

VL - 16

SP - 840

EP - 843

JO - Cancer Epidemiology Biomarkers and Prevention

JF - Cancer Epidemiology Biomarkers and Prevention

SN - 1055-9965

IS - 4

ER -